Gene discovery in Plasmodium vivax through sequencing of ESTs from mixed blood stages

Liwang Cui, Qi Fan, Yi Hu, Svetlana A. Karamycheva, John Quackenbush, Benjawan Khuntirat, Jetsumon Sattabongkot, Jane M. Carlton

Research output: Contribution to journalArticle

Abstract

Despite the significance of Plasmodium vivax as the most widespread human malaria parasite and a major public health problem, gene expression in this parasite is poorly understood. To accelerate gene discovery and facilitate the annotation phase of the P. vivax genome project, we have undertaken a transcriptome approach to study gene expression in the mixed blood stages of a P. vivax field isolate. Using a cDNA library constructed from purified blood stages, we have obtained single-pass sequences for approximately 21,500 expressed sequence tags (ESTs), the largest number of transcript tags obtained so far for this species. Cluster analysis revealed that the library is highly redundant, resulting in 5407 clusters. Clustered ESTs were searched against public protein databases for functional annotation, and more than one-third showed a significant match, the majority of these to Plasmodium falciparum proteins. The most abundant clusters were to genes encoding ribosomal proteins and proteins involved in metabolism, consistent with the predominance of trophozoites in the field isolate sample. In spite of the scarcity of other parasite stages in the field isolate, we could identify genes that are expressed in rings, schizonts and gametocytes. This study should facilitate our understanding of the gene expression in P. vivax asexual stages and provide valuable data for gene prediction and annotation of the P. vivax genome sequence.

Original languageEnglish (US)
Pages (from-to)1-9
Number of pages9
JournalMolecular and Biochemical Parasitology
Volume144
Issue number1
DOIs
StatePublished - Nov 2005

Fingerprint

Plasmodium vivax
Expressed Sequence Tags
Genetic Association Studies
Parasites
Gene Expression
Genome
Schizonts
Molecular Sequence Annotation
Trophozoites
Protein Databases
Ribosomal Proteins
Plasmodium falciparum
Gene Library
Transcriptome
Genes
Malaria
Libraries
Cluster Analysis
Proteins
Public Health

Keywords

  • Blood stages
  • cDNA library
  • EST
  • Gene expression
  • Genomics
  • Malaria parasite

ASJC Scopus subject areas

  • Molecular Biology
  • Parasitology

Cite this

Gene discovery in Plasmodium vivax through sequencing of ESTs from mixed blood stages. / Cui, Liwang; Fan, Qi; Hu, Yi; Karamycheva, Svetlana A.; Quackenbush, John; Khuntirat, Benjawan; Sattabongkot, Jetsumon; Carlton, Jane M.

In: Molecular and Biochemical Parasitology, Vol. 144, No. 1, 11.2005, p. 1-9.

Research output: Contribution to journalArticle

Cui, L, Fan, Q, Hu, Y, Karamycheva, SA, Quackenbush, J, Khuntirat, B, Sattabongkot, J & Carlton, JM 2005, 'Gene discovery in Plasmodium vivax through sequencing of ESTs from mixed blood stages', Molecular and Biochemical Parasitology, vol. 144, no. 1, pp. 1-9. https://doi.org/10.1016/j.molbiopara.2005.05.016
Cui, Liwang ; Fan, Qi ; Hu, Yi ; Karamycheva, Svetlana A. ; Quackenbush, John ; Khuntirat, Benjawan ; Sattabongkot, Jetsumon ; Carlton, Jane M. / Gene discovery in Plasmodium vivax through sequencing of ESTs from mixed blood stages. In: Molecular and Biochemical Parasitology. 2005 ; Vol. 144, No. 1. pp. 1-9.
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