GCFIL-1β profiles in periodontal disease

Steven Engebretson, John T. Grbic, Robert Singer, Ira B. Lamster

Research output: Contribution to journalArticle

Abstract

Objectives: Studies suggest a genetic influence on levels of interleukin-1β (IL-1β) in gingival crevicular fluid (GCF). Levels of IL-1β in GCF, however, are also dependent upon the clinical parameters at the site of collection, including probing depth (PD) and level of attachment (AL). To examine this issue, IL-1β in GCF was evaluated from patients with varying degrees of periodontal disease. The influence of both the status of the patient and the probing depth at the sampled sites were considered in the analysis. Material and methods: GCF IL-1β was determined by ELISA at 6-8 molar sites from 29 non-smoking adults with mild, moderate, or severe periodontal disease at baseline, 2 weeks, and 24 weeks following scaling and root planing. For later analysis, patients were dichotomized on the basis of disease severity (mild/moderate vs severe). Sampled sites were classified at baseline by PD as, shallow (<4 mm), intermediate (4-6 mm), or deep (>6 mm). Results: PD and AL were each strongly correlated with IL-1β levels at baseline. However, patients with severe disease had higher levels of IL-1β in each PD category than those with mild/moderate disease. As compared to patients with mild/ moderate disease, IL-1β levels in shallow sites from patients with severe disease was elevated nearly 2 fold (p<0.001). IL-1β levels were reduced in all groups at 2 weeks and were still significantly reduced in patients with mild/moderate disease at 24 weeks. At 24 weeks IL-1β returned to near baseline levels in patients with severe disease. Conclusion: While PD and AL are each associated with increased GCF IL-1β, patients with severe disease show higher IL-1β GCF levels in shallow sites, suggesting that high GCF IL-1β expression is in part a host trait, and not strictly a function of clinical parameters.

Original languageEnglish (US)
Pages (from-to)48-53
Number of pages6
JournalJournal of Clinical Periodontology
Volume29
Issue number1
DOIs
StatePublished - Jan 2002

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Periodontal Diseases
Interleukin-1
Gingival Crevicular Fluid
Root Planing
Enzyme-Linked Immunosorbent Assay

Keywords

  • Adult
  • Analysis of variance
  • Crevicular fluid
  • Interteukin-1β
  • Periodontal therapy
  • Periodontitis

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

GCFIL-1β profiles in periodontal disease. / Engebretson, Steven; Grbic, John T.; Singer, Robert; Lamster, Ira B.

In: Journal of Clinical Periodontology, Vol. 29, No. 1, 01.2002, p. 48-53.

Research output: Contribution to journalArticle

Engebretson, Steven ; Grbic, John T. ; Singer, Robert ; Lamster, Ira B. / GCFIL-1β profiles in periodontal disease. In: Journal of Clinical Periodontology. 2002 ; Vol. 29, No. 1. pp. 48-53.
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abstract = "Objectives: Studies suggest a genetic influence on levels of interleukin-1β (IL-1β) in gingival crevicular fluid (GCF). Levels of IL-1β in GCF, however, are also dependent upon the clinical parameters at the site of collection, including probing depth (PD) and level of attachment (AL). To examine this issue, IL-1β in GCF was evaluated from patients with varying degrees of periodontal disease. The influence of both the status of the patient and the probing depth at the sampled sites were considered in the analysis. Material and methods: GCF IL-1β was determined by ELISA at 6-8 molar sites from 29 non-smoking adults with mild, moderate, or severe periodontal disease at baseline, 2 weeks, and 24 weeks following scaling and root planing. For later analysis, patients were dichotomized on the basis of disease severity (mild/moderate vs severe). Sampled sites were classified at baseline by PD as, shallow (<4 mm), intermediate (4-6 mm), or deep (>6 mm). Results: PD and AL were each strongly correlated with IL-1β levels at baseline. However, patients with severe disease had higher levels of IL-1β in each PD category than those with mild/moderate disease. As compared to patients with mild/ moderate disease, IL-1β levels in shallow sites from patients with severe disease was elevated nearly 2 fold (p<0.001). IL-1β levels were reduced in all groups at 2 weeks and were still significantly reduced in patients with mild/moderate disease at 24 weeks. At 24 weeks IL-1β returned to near baseline levels in patients with severe disease. Conclusion: While PD and AL are each associated with increased GCF IL-1β, patients with severe disease show higher IL-1β GCF levels in shallow sites, suggesting that high GCF IL-1β expression is in part a host trait, and not strictly a function of clinical parameters.",
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