Gγ13 colocalizes with gustducin in taste receptor cells and mediates IP3 responses to bitter denatonium

Liquan Huangu, Y. Gopi Shanker, Jolanta Dubauskaite, Jenny Z. Zheng, Wentao Yan, Sophia Rosenweig, Andrew Spielman, Marianna Max, Robert F. Margolskee

Research output: Contribution to journalArticle

Abstract

Gustducin is a transducin-like G protein selectively expressed in taste receptor cells. The α subunit of gustducin (α-gustducin) is critical for transduction of responses to bitter or sweet compounds. We identified a G- protein γ subunit (Gγ13) that colocalized with α-gustducin in taste receptor cells. Of 19 α-gustducin/Gγ13-positive taste receptor cells profiled, all expressed the G protein β3 subunit (Gβ3); ~80% also expressed Gβ1. Gustducin heterotrimers (α-gustducin/Gβ1/Gγ13) were activated by taste cell membranes plus bitter denatonium. Antibodies against Gγ13 blocked the denatonium-induced increase of inositol trisphosphate (IP3) in taste tissue. We conclude that gustducin heterotrimers transduce responses to bitter and sweet compounds via α-gustducin's regulation of phosphodiesterase (PDE) and Gβγ's activation of phospholipase C (PLC).

Original languageEnglish (US)
Pages (from-to)1055-1062
Number of pages8
JournalNature Neuroscience
Volume2
Issue number12
DOIs
StatePublished - Dec 1999

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GTP-Binding Proteins
G12-G13 GTP-Binding Protein alpha Subunits
Transducin
gustducin
Phosphoric Diester Hydrolases
Protein Subunits
Type C Phospholipases
Inositol
Cell Membrane
Antibodies

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Huangu, L., Shanker, Y. G., Dubauskaite, J., Zheng, J. Z., Yan, W., Rosenweig, S., ... Margolskee, R. F. (1999). Gγ13 colocalizes with gustducin in taste receptor cells and mediates IP3 responses to bitter denatonium. Nature Neuroscience, 2(12), 1055-1062. https://doi.org/10.1038/15981

Gγ13 colocalizes with gustducin in taste receptor cells and mediates IP3 responses to bitter denatonium. / Huangu, Liquan; Shanker, Y. Gopi; Dubauskaite, Jolanta; Zheng, Jenny Z.; Yan, Wentao; Rosenweig, Sophia; Spielman, Andrew; Max, Marianna; Margolskee, Robert F.

In: Nature Neuroscience, Vol. 2, No. 12, 12.1999, p. 1055-1062.

Research output: Contribution to journalArticle

Huangu, L, Shanker, YG, Dubauskaite, J, Zheng, JZ, Yan, W, Rosenweig, S, Spielman, A, Max, M & Margolskee, RF 1999, 'Gγ13 colocalizes with gustducin in taste receptor cells and mediates IP3 responses to bitter denatonium', Nature Neuroscience, vol. 2, no. 12, pp. 1055-1062. https://doi.org/10.1038/15981
Huangu L, Shanker YG, Dubauskaite J, Zheng JZ, Yan W, Rosenweig S et al. Gγ13 colocalizes with gustducin in taste receptor cells and mediates IP3 responses to bitter denatonium. Nature Neuroscience. 1999 Dec;2(12):1055-1062. https://doi.org/10.1038/15981
Huangu, Liquan ; Shanker, Y. Gopi ; Dubauskaite, Jolanta ; Zheng, Jenny Z. ; Yan, Wentao ; Rosenweig, Sophia ; Spielman, Andrew ; Max, Marianna ; Margolskee, Robert F. / Gγ13 colocalizes with gustducin in taste receptor cells and mediates IP3 responses to bitter denatonium. In: Nature Neuroscience. 1999 ; Vol. 2, No. 12. pp. 1055-1062.
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