Functional and geographical differentiation of candidate balanced polymorphisms in Arabidopsis thaliana

Jennifer M. Reininga, Dahlia Nielsen, Michael D. Purugganan

Research output: Contribution to journalArticle

Abstract

Molecular population genetic analysis of three chromosomal regions in Arabidopsis thaliana suggested that balancing selection might operate to maintain variation at three novel candidate adaptive trait genes, including SOLUBLE STARCH SYNTHASE I (SSI), PLASTID TRANSCRIPTIONALLY ACTIVE 7(PTAC7), and BELL-LIKE HOMEODOMAIN 10 (BLH10). If balanced polymorphisms are indeed maintained at these loci, then we would expect to observe functional variation underlying the previously detected signatures of selection. We observe multiple replacement polymorphisms within and in the 32 amino acids just upstream of the protein-protein interacting BELL domain at the BLH10 locus. While no clear protein sequence differences are found between allele types in SSI and PTAC7, these two genes show evidence for allele-specific variation in expression levels. Geographical patterns of allelic differentiation seem consistent with population stratification in this species and a significant longitudinal cline was observed at all three candidate loci. These data support a hypothesis of balancing selection at all three candidate loci and provide a basis for more detailed functional work by identifying possible functional differences that might be selectively maintained.

Original languageEnglish (US)
Pages (from-to)2844-2855
Number of pages12
JournalMolecular Ecology
Volume18
Issue number13
DOIs
StatePublished - Jul 2009

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Arabidopsis
Glycogen Synthase
polymorphism
Plastids
Arabidopsis thaliana
plastid
genetic polymorphism
starch
Starch
starch synthase
loci
protein
allele
Alleles
plastids
Proteins
cline
gene
Population Genetics
genetic analysis

Keywords

  • Adaptation
  • Balancing selection
  • Population genetics

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

Cite this

Functional and geographical differentiation of candidate balanced polymorphisms in Arabidopsis thaliana. / Reininga, Jennifer M.; Nielsen, Dahlia; Purugganan, Michael D.

In: Molecular Ecology, Vol. 18, No. 13, 07.2009, p. 2844-2855.

Research output: Contribution to journalArticle

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