Functional analyses of the promoters in the lantibiotic mutacin II biosynthetic locus in Streptococcus mutans

Fengxia Qi, Ping Chen, Page W. Caufield

Research output: Contribution to journalArticle

Abstract

The lantibiotic bacteriocin mutacin II is produced by the group II Streptococcus mutans. The mutacin II biosynthetic locus consists of seven genes, mutR, -A, -M, -T, -F, -E, and -G, organized as two operons. The mutAMTFEG operon is transcribed from the mutA promoter 55 bp upstream of the translation start codon for MutA, while the mutR promoter is 76 bp upstream of the mutR structural gene. Expression of the mutA promoter is regulated by the components of the growth medium, while the mutR promoter activity does not seem to be affected by these conditions. Inactivation of mutR abolishes transcription of the mutA operon but does not affect its own promoter activity. The expressions of both mutA and mutR promoters are independent of the growth stage, while the production of mutacin II is only elevated at the early stationary phase. Taken together, these results suggest that expression of the mutacin operon is regulated by a complex system involving transcriptional and posttranscriptional or posttranslational controls.

Original languageEnglish (US)
Pages (from-to)652-658
Number of pages7
JournalApplied and Environmental Microbiology
Volume65
Issue number2
StatePublished - Feb 1999

Fingerprint

Streptococcus mutans
Bacteriocins
bacteriocins
Operon
promoter regions
operon
loci
gene
Initiator Codon
Growth
Genes
start codon
structural genes
translation (genetics)
mutacin II
inactivation
transcription (genetics)
culture media
developmental stages

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

Functional analyses of the promoters in the lantibiotic mutacin II biosynthetic locus in Streptococcus mutans. / Qi, Fengxia; Chen, Ping; Caufield, Page W.

In: Applied and Environmental Microbiology, Vol. 65, No. 2, 02.1999, p. 652-658.

Research output: Contribution to journalArticle

@article{957c92b4a438420bb2ef8f73bb8b24a5,
title = "Functional analyses of the promoters in the lantibiotic mutacin II biosynthetic locus in Streptococcus mutans",
abstract = "The lantibiotic bacteriocin mutacin II is produced by the group II Streptococcus mutans. The mutacin II biosynthetic locus consists of seven genes, mutR, -A, -M, -T, -F, -E, and -G, organized as two operons. The mutAMTFEG operon is transcribed from the mutA promoter 55 bp upstream of the translation start codon for MutA, while the mutR promoter is 76 bp upstream of the mutR structural gene. Expression of the mutA promoter is regulated by the components of the growth medium, while the mutR promoter activity does not seem to be affected by these conditions. Inactivation of mutR abolishes transcription of the mutA operon but does not affect its own promoter activity. The expressions of both mutA and mutR promoters are independent of the growth stage, while the production of mutacin II is only elevated at the early stationary phase. Taken together, these results suggest that expression of the mutacin operon is regulated by a complex system involving transcriptional and posttranscriptional or posttranslational controls.",
author = "Fengxia Qi and Ping Chen and Caufield, {Page W.}",
year = "1999",
month = "2",
language = "English (US)",
volume = "65",
pages = "652--658",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
number = "2",

}

TY - JOUR

T1 - Functional analyses of the promoters in the lantibiotic mutacin II biosynthetic locus in Streptococcus mutans

AU - Qi, Fengxia

AU - Chen, Ping

AU - Caufield, Page W.

PY - 1999/2

Y1 - 1999/2

N2 - The lantibiotic bacteriocin mutacin II is produced by the group II Streptococcus mutans. The mutacin II biosynthetic locus consists of seven genes, mutR, -A, -M, -T, -F, -E, and -G, organized as two operons. The mutAMTFEG operon is transcribed from the mutA promoter 55 bp upstream of the translation start codon for MutA, while the mutR promoter is 76 bp upstream of the mutR structural gene. Expression of the mutA promoter is regulated by the components of the growth medium, while the mutR promoter activity does not seem to be affected by these conditions. Inactivation of mutR abolishes transcription of the mutA operon but does not affect its own promoter activity. The expressions of both mutA and mutR promoters are independent of the growth stage, while the production of mutacin II is only elevated at the early stationary phase. Taken together, these results suggest that expression of the mutacin operon is regulated by a complex system involving transcriptional and posttranscriptional or posttranslational controls.

AB - The lantibiotic bacteriocin mutacin II is produced by the group II Streptococcus mutans. The mutacin II biosynthetic locus consists of seven genes, mutR, -A, -M, -T, -F, -E, and -G, organized as two operons. The mutAMTFEG operon is transcribed from the mutA promoter 55 bp upstream of the translation start codon for MutA, while the mutR promoter is 76 bp upstream of the mutR structural gene. Expression of the mutA promoter is regulated by the components of the growth medium, while the mutR promoter activity does not seem to be affected by these conditions. Inactivation of mutR abolishes transcription of the mutA operon but does not affect its own promoter activity. The expressions of both mutA and mutR promoters are independent of the growth stage, while the production of mutacin II is only elevated at the early stationary phase. Taken together, these results suggest that expression of the mutacin operon is regulated by a complex system involving transcriptional and posttranscriptional or posttranslational controls.

UR - http://www.scopus.com/inward/record.url?scp=0033051428&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033051428&partnerID=8YFLogxK

M3 - Article

VL - 65

SP - 652

EP - 658

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 2

ER -