FoxF is essential for FGF-induced migration of heart progenitor cells in the ascidian Ciona intestinalis

Jeni Beh, Weiyang Shi, Mike Levine, Brad Davidson, Lionel Christiaen

Research output: Contribution to journalArticle

Abstract

Heart development requires precise coordination of morphogenetic movements with progressive cell fate specification and differentiation. In ascidian embryos, FGF/MAPK-mediated activation of the transcription factor Ets1/2 is required for heart tissue specification and cell migration. We found that FoxF is one of the first genes to be activated in heart precursors in response to FGF signaling. We identified the FoxF minimal heart enhancer and used a cis-trans complementation test to show that Ets1/2 can interact with the FoxF enhancer in vivo. Next, we found that FoxF function is required downstream and in parallel to the FGF/ MAPK/Ets cascade for cell migration. In addition, we demonstrated that targeted expression of a dominant-negative form of FoxF inhibits cell migration but not heart differentiation, resulting in a striking phenotype: a beating heart at an ectopic location within the body cavity of juveniles. Taken together, our results indicate that FoxF is a direct target of FGF signaling and is predominantly involved in the regulation of heart cell migration.

Original languageEnglish (US)
Pages (from-to)3297-3305
Number of pages9
JournalDevelopment
Volume134
Issue number18
DOIs
StatePublished - Sep 2007

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Ciona intestinalis
Urochordata
Stem Cells
Cell Movement
Genetic Complementation Test
Transcription Factors
Embryonic Structures
Phenotype

Keywords

  • Ascidian
  • Cardiac morphogenesis
  • Directed cell migration
  • FGF signaling
  • Forkhead

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

FoxF is essential for FGF-induced migration of heart progenitor cells in the ascidian Ciona intestinalis. / Beh, Jeni; Shi, Weiyang; Levine, Mike; Davidson, Brad; Christiaen, Lionel.

In: Development, Vol. 134, No. 18, 09.2007, p. 3297-3305.

Research output: Contribution to journalArticle

Beh, Jeni ; Shi, Weiyang ; Levine, Mike ; Davidson, Brad ; Christiaen, Lionel. / FoxF is essential for FGF-induced migration of heart progenitor cells in the ascidian Ciona intestinalis. In: Development. 2007 ; Vol. 134, No. 18. pp. 3297-3305.
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