Fluorescence depolarization study of randomly oriented and magneto-oriented spinach chloroplasts in suspension

J. Breton, J. F. Becker, Nicholas Geacintov

Research output: Contribution to journalArticle

Abstract

The degree of polarization of fluorescence (P) of chlorophyll a in vivo has been measured using suspensions of either randomly oriented or magnetically oriented spinach chloroplasts. In the latter case the viewing direction was perpendicular to the plane of the oriented photosynthetic membranes. P decreases from 3.5% (unoriented) to 1.5% when the chloroplasts are oriented in a 13 Kgauss magnetic field. This decrease can be explained by the anisotropic optical properties of the membranes. The residual 1.5% polarization is shown to reflect the depolarization by energy transfer. Similar effects have been observed with Chlorella.

Original languageEnglish (US)
Pages (from-to)1403-1409
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume54
Issue number4
DOIs
StatePublished - Oct 15 1973

Fingerprint

Spinacia oleracea
Depolarization
Chloroplasts
Photosynthetic membranes
Suspensions
Fluorescence
Polarization
Chlorella
Fluorescence Polarization
Membranes
Energy Transfer
Magnetic Fields
Energy transfer
Optical properties
Magnetic fields
chlorophyll a
Direction compound

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Fluorescence depolarization study of randomly oriented and magneto-oriented spinach chloroplasts in suspension. / Breton, J.; Becker, J. F.; Geacintov, Nicholas.

In: Biochemical and Biophysical Research Communications, Vol. 54, No. 4, 15.10.1973, p. 1403-1409.

Research output: Contribution to journalArticle

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