Fluorescence-activated cell sorting for analysis of cell type-specific responses to salinity stress in Arabidopsis and rice.

Aurelie Evrard, Bastiaan O R Bargmann, Kenneth D. Birnbaum, Mark Tester, Ute Baumann, Alexander A T Johnson

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Fluorescence-activated cell sorting (FACS) provides a rapid means of isolating large numbers of fluorescently tagged cells from a heterogeneous mixture of cells. Collections of transgenic plants with cell type-specific expression of fluorescent marker genes such as green fluorescent protein (GFP) are ideally suited for FACS-assisted studies of individual cell types. Here we describe the use of Arabidopsis and rice enhancer trap lines with tissue-specific GFP expression patterns in the root to isolate specific cell types of root tissues using FACS. Additionally, protocols are provided to impose a ramped salinity stress for 48 h prior to cell sorting.

Original languageEnglish (US)
Title of host publicationPlant Salt Tolerance: Methods and Protocols
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages265-276
Number of pages12
Volume913
ISBN (Print)9781617799853
DOIs
StatePublished - 2012

Publication series

NameMethods in Molecular Biology
Volume913
ISSN (Print)1064-3745

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Keywords

  • Arabidopsis
  • Cell type-specific
  • FACS
  • GAL4
  • Protoplast
  • Rice
  • Salinity

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Evrard, A., Bargmann, B. O. R., Birnbaum, K. D., Tester, M., Baumann, U., & Johnson, A. A. T. (2012). Fluorescence-activated cell sorting for analysis of cell type-specific responses to salinity stress in Arabidopsis and rice. In Plant Salt Tolerance: Methods and Protocols: Methods and Protocols (Vol. 913, pp. 265-276). (Methods in Molecular Biology; Vol. 913). Humana Press Inc.. https://doi.org/10.1007/978-1-61779-986-0_18