Flow visualization and flow cytometry with holographic video microscopy

Fook Chiong Cheong, Bo Sun, Remi Dreyfus, Jesse Amato-Grill, Ke Xiao, Lisa Dixon, David G. Grier

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    The video stream captured by an in-line holographic microscope can be analyzed on a frame-by-frame basis to track individual colloidal particles' three-dimensional motions with nanometer resolution, and simultaneously to measure their sizes and refractive indexes. An efficient particle-tracking algorithm automates initial position estimation with sufficient accuracy to enable unattended holographic particle tracking and characterization. In this work, we demonstrated this approach to flow visualization in a microfluidic channel and also to flow cytometry of micrometer-scale colloidal spheres.

    Original languageEnglish (US)
    Title of host publicationPractical Holography XXIV: Materials and Applications
    Volume7619
    DOIs
    StatePublished - 2010
    EventPractical Holography XXIV: Materials and Applications - San Francisco, CA, United States
    Duration: Jan 24 2010Jan 27 2010

    Other

    OtherPractical Holography XXIV: Materials and Applications
    CountryUnited States
    CitySan Francisco, CA
    Period1/24/101/27/10

    Fingerprint

    Flow Visualization
    Flow Cytometry
    Particle Tracking
    cytometry
    Flow cytometry
    flow visualization
    Flow visualization
    Microfluidics
    Microscopy
    Refractive index
    Microscopic examination
    Microscopes
    microscopy
    Microscope
    three dimensional motion
    Refractive Index
    Sufficient
    Three-dimensional
    Motion
    micrometers

    Keywords

    • Cytometry
    • Digital Holography
    • Particle tracking

    ASJC Scopus subject areas

    • Applied Mathematics
    • Computer Science Applications
    • Electrical and Electronic Engineering
    • Electronic, Optical and Magnetic Materials
    • Condensed Matter Physics

    Cite this

    Cheong, F. C., Sun, B., Dreyfus, R., Amato-Grill, J., Xiao, K., Dixon, L., & Grier, D. G. (2010). Flow visualization and flow cytometry with holographic video microscopy. In Practical Holography XXIV: Materials and Applications (Vol. 7619). [761905] https://doi.org/10.1117/12.840550

    Flow visualization and flow cytometry with holographic video microscopy. / Cheong, Fook Chiong; Sun, Bo; Dreyfus, Remi; Amato-Grill, Jesse; Xiao, Ke; Dixon, Lisa; Grier, David G.

    Practical Holography XXIV: Materials and Applications. Vol. 7619 2010. 761905.

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Cheong, FC, Sun, B, Dreyfus, R, Amato-Grill, J, Xiao, K, Dixon, L & Grier, DG 2010, Flow visualization and flow cytometry with holographic video microscopy. in Practical Holography XXIV: Materials and Applications. vol. 7619, 761905, Practical Holography XXIV: Materials and Applications, San Francisco, CA, United States, 1/24/10. https://doi.org/10.1117/12.840550
    Cheong FC, Sun B, Dreyfus R, Amato-Grill J, Xiao K, Dixon L et al. Flow visualization and flow cytometry with holographic video microscopy. In Practical Holography XXIV: Materials and Applications. Vol. 7619. 2010. 761905 https://doi.org/10.1117/12.840550
    Cheong, Fook Chiong ; Sun, Bo ; Dreyfus, Remi ; Amato-Grill, Jesse ; Xiao, Ke ; Dixon, Lisa ; Grier, David G. / Flow visualization and flow cytometry with holographic video microscopy. Practical Holography XXIV: Materials and Applications. Vol. 7619 2010.
    @inproceedings{2f52e0018c5246618d75e5baf5ffc5ca,
    title = "Flow visualization and flow cytometry with holographic video microscopy",
    abstract = "The video stream captured by an in-line holographic microscope can be analyzed on a frame-by-frame basis to track individual colloidal particles' three-dimensional motions with nanometer resolution, and simultaneously to measure their sizes and refractive indexes. An efficient particle-tracking algorithm automates initial position estimation with sufficient accuracy to enable unattended holographic particle tracking and characterization. In this work, we demonstrated this approach to flow visualization in a microfluidic channel and also to flow cytometry of micrometer-scale colloidal spheres.",
    keywords = "Cytometry, Digital Holography, Particle tracking",
    author = "Cheong, {Fook Chiong} and Bo Sun and Remi Dreyfus and Jesse Amato-Grill and Ke Xiao and Lisa Dixon and Grier, {David G.}",
    year = "2010",
    doi = "10.1117/12.840550",
    language = "English (US)",
    isbn = "9780819480156",
    volume = "7619",
    booktitle = "Practical Holography XXIV: Materials and Applications",

    }

    TY - GEN

    T1 - Flow visualization and flow cytometry with holographic video microscopy

    AU - Cheong, Fook Chiong

    AU - Sun, Bo

    AU - Dreyfus, Remi

    AU - Amato-Grill, Jesse

    AU - Xiao, Ke

    AU - Dixon, Lisa

    AU - Grier, David G.

    PY - 2010

    Y1 - 2010

    N2 - The video stream captured by an in-line holographic microscope can be analyzed on a frame-by-frame basis to track individual colloidal particles' three-dimensional motions with nanometer resolution, and simultaneously to measure their sizes and refractive indexes. An efficient particle-tracking algorithm automates initial position estimation with sufficient accuracy to enable unattended holographic particle tracking and characterization. In this work, we demonstrated this approach to flow visualization in a microfluidic channel and also to flow cytometry of micrometer-scale colloidal spheres.

    AB - The video stream captured by an in-line holographic microscope can be analyzed on a frame-by-frame basis to track individual colloidal particles' three-dimensional motions with nanometer resolution, and simultaneously to measure their sizes and refractive indexes. An efficient particle-tracking algorithm automates initial position estimation with sufficient accuracy to enable unattended holographic particle tracking and characterization. In this work, we demonstrated this approach to flow visualization in a microfluidic channel and also to flow cytometry of micrometer-scale colloidal spheres.

    KW - Cytometry

    KW - Digital Holography

    KW - Particle tracking

    UR - http://www.scopus.com/inward/record.url?scp=77951689555&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=77951689555&partnerID=8YFLogxK

    U2 - 10.1117/12.840550

    DO - 10.1117/12.840550

    M3 - Conference contribution

    SN - 9780819480156

    VL - 7619

    BT - Practical Holography XXIV: Materials and Applications

    ER -