First trimester vitamin D status and placental epigenomics in preeclampsia among Northern Plains primiparas

Cindy M. Anderson, Jody L. Ralph, Luann Johnson, Angela Scheett, Michelle L. Wright, Jacquelyn Taylor, Joyce E. Ohm, Eric Uthus

Research output: Contribution to journalArticle

Abstract

Aims As maternal vitamin D status has been associated with preeclampsia, the purpose of this study was to determine variations in DNA methylation patterns and associated protein expression in placental genes regulating vitamin D metabolism. Main methods A convenience sample of 48 pregnant nulliparous women, including 11 later diagnosed with preeclampsia, were recruited in this prospective study. Using a case-control design in two groups of women, we administered a food frequency questionnaire to determine vitamin D dietary intake. Laboratory measures included serum vitamin D levels (25[OH]D), DNA methylation patterns and protein expression in placental genes regulating vitamin D metabolism (1α-hydroxylase, CYP27B1; vitamin D receptor, VDR; retinoid X receptor, RXR) from placental tissue collected at delivery among those diagnosed with preeclampsia and those who remained normotensive throughout pregnancy. Key findings There were no significant differences in vitamin D dietary intake or mean serum 25[OH]D levels, although the proportion of women with deficient 25[OH]D levels was higher in the preeclampsia group (46%) than the normotensive group (20%). Placenta samples from women with preeclampsia also had increased DNA methylation of CYP27B1, VDR and RXR genes with lower protein expression levels limited to RXR. Significance Hypermethylation of key placental genes involved in vitamin D metabolism suggests uncoupling of processes that may interfere with placentation and availability of vitamin D at the maternal-fetal interface.

Original languageEnglish (US)
Pages (from-to)10-15
Number of pages6
JournalLife Sciences
Volume129
DOIs
StatePublished - May 15 2015

Fingerprint

First Pregnancy Trimester
Pre-Eclampsia
Epigenomics
Vitamin D
25-Hydroxyvitamin D3 1-alpha-Hydroxylase
DNA Methylation
Genes
Metabolism
Mothers
Retinoid X Receptors
Placentation
Calcitriol Receptors
Proteins
Mixed Function Oxygenases
Serum
Placenta
Pregnant Women
Availability
Prospective Studies
Tissue

Keywords

  • DNA methylation
  • Gestational hypertension
  • Vitamin D

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

First trimester vitamin D status and placental epigenomics in preeclampsia among Northern Plains primiparas. / Anderson, Cindy M.; Ralph, Jody L.; Johnson, Luann; Scheett, Angela; Wright, Michelle L.; Taylor, Jacquelyn; Ohm, Joyce E.; Uthus, Eric.

In: Life Sciences, Vol. 129, 15.05.2015, p. 10-15.

Research output: Contribution to journalArticle

Anderson, Cindy M. ; Ralph, Jody L. ; Johnson, Luann ; Scheett, Angela ; Wright, Michelle L. ; Taylor, Jacquelyn ; Ohm, Joyce E. ; Uthus, Eric. / First trimester vitamin D status and placental epigenomics in preeclampsia among Northern Plains primiparas. In: Life Sciences. 2015 ; Vol. 129. pp. 10-15.
@article{4b5fac4f266b4490a6b5d49d95978fd4,
title = "First trimester vitamin D status and placental epigenomics in preeclampsia among Northern Plains primiparas",
abstract = "Aims As maternal vitamin D status has been associated with preeclampsia, the purpose of this study was to determine variations in DNA methylation patterns and associated protein expression in placental genes regulating vitamin D metabolism. Main methods A convenience sample of 48 pregnant nulliparous women, including 11 later diagnosed with preeclampsia, were recruited in this prospective study. Using a case-control design in two groups of women, we administered a food frequency questionnaire to determine vitamin D dietary intake. Laboratory measures included serum vitamin D levels (25[OH]D), DNA methylation patterns and protein expression in placental genes regulating vitamin D metabolism (1α-hydroxylase, CYP27B1; vitamin D receptor, VDR; retinoid X receptor, RXR) from placental tissue collected at delivery among those diagnosed with preeclampsia and those who remained normotensive throughout pregnancy. Key findings There were no significant differences in vitamin D dietary intake or mean serum 25[OH]D levels, although the proportion of women with deficient 25[OH]D levels was higher in the preeclampsia group (46{\%}) than the normotensive group (20{\%}). Placenta samples from women with preeclampsia also had increased DNA methylation of CYP27B1, VDR and RXR genes with lower protein expression levels limited to RXR. Significance Hypermethylation of key placental genes involved in vitamin D metabolism suggests uncoupling of processes that may interfere with placentation and availability of vitamin D at the maternal-fetal interface.",
keywords = "DNA methylation, Gestational hypertension, Vitamin D",
author = "Anderson, {Cindy M.} and Ralph, {Jody L.} and Luann Johnson and Angela Scheett and Wright, {Michelle L.} and Jacquelyn Taylor and Ohm, {Joyce E.} and Eric Uthus",
year = "2015",
month = "5",
day = "15",
doi = "10.1016/j.lfs.2014.07.012",
language = "English (US)",
volume = "129",
pages = "10--15",
journal = "Life Sciences",
issn = "0024-3205",
publisher = "Elsevier Inc.",

}

TY - JOUR

T1 - First trimester vitamin D status and placental epigenomics in preeclampsia among Northern Plains primiparas

AU - Anderson, Cindy M.

AU - Ralph, Jody L.

AU - Johnson, Luann

AU - Scheett, Angela

AU - Wright, Michelle L.

AU - Taylor, Jacquelyn

AU - Ohm, Joyce E.

AU - Uthus, Eric

PY - 2015/5/15

Y1 - 2015/5/15

N2 - Aims As maternal vitamin D status has been associated with preeclampsia, the purpose of this study was to determine variations in DNA methylation patterns and associated protein expression in placental genes regulating vitamin D metabolism. Main methods A convenience sample of 48 pregnant nulliparous women, including 11 later diagnosed with preeclampsia, were recruited in this prospective study. Using a case-control design in two groups of women, we administered a food frequency questionnaire to determine vitamin D dietary intake. Laboratory measures included serum vitamin D levels (25[OH]D), DNA methylation patterns and protein expression in placental genes regulating vitamin D metabolism (1α-hydroxylase, CYP27B1; vitamin D receptor, VDR; retinoid X receptor, RXR) from placental tissue collected at delivery among those diagnosed with preeclampsia and those who remained normotensive throughout pregnancy. Key findings There were no significant differences in vitamin D dietary intake or mean serum 25[OH]D levels, although the proportion of women with deficient 25[OH]D levels was higher in the preeclampsia group (46%) than the normotensive group (20%). Placenta samples from women with preeclampsia also had increased DNA methylation of CYP27B1, VDR and RXR genes with lower protein expression levels limited to RXR. Significance Hypermethylation of key placental genes involved in vitamin D metabolism suggests uncoupling of processes that may interfere with placentation and availability of vitamin D at the maternal-fetal interface.

AB - Aims As maternal vitamin D status has been associated with preeclampsia, the purpose of this study was to determine variations in DNA methylation patterns and associated protein expression in placental genes regulating vitamin D metabolism. Main methods A convenience sample of 48 pregnant nulliparous women, including 11 later diagnosed with preeclampsia, were recruited in this prospective study. Using a case-control design in two groups of women, we administered a food frequency questionnaire to determine vitamin D dietary intake. Laboratory measures included serum vitamin D levels (25[OH]D), DNA methylation patterns and protein expression in placental genes regulating vitamin D metabolism (1α-hydroxylase, CYP27B1; vitamin D receptor, VDR; retinoid X receptor, RXR) from placental tissue collected at delivery among those diagnosed with preeclampsia and those who remained normotensive throughout pregnancy. Key findings There were no significant differences in vitamin D dietary intake or mean serum 25[OH]D levels, although the proportion of women with deficient 25[OH]D levels was higher in the preeclampsia group (46%) than the normotensive group (20%). Placenta samples from women with preeclampsia also had increased DNA methylation of CYP27B1, VDR and RXR genes with lower protein expression levels limited to RXR. Significance Hypermethylation of key placental genes involved in vitamin D metabolism suggests uncoupling of processes that may interfere with placentation and availability of vitamin D at the maternal-fetal interface.

KW - DNA methylation

KW - Gestational hypertension

KW - Vitamin D

UR - http://www.scopus.com/inward/record.url?scp=84939892808&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84939892808&partnerID=8YFLogxK

U2 - 10.1016/j.lfs.2014.07.012

DO - 10.1016/j.lfs.2014.07.012

M3 - Article

VL - 129

SP - 10

EP - 15

JO - Life Sciences

JF - Life Sciences

SN - 0024-3205

ER -