Fetal cleft lip repair in rabbits

Histology and role of hyaluronic acid

Michael Stern, Brian Schmidt, Thomas B. Dodson, Robert Stern, Leonard B. Kaban

Research output: Contribution to journalArticle

Abstract

This study examines the histologic and biochemical features of wound healing in a cleft lip model in the mid-third-trimester fetal rabbit. At days 1, 2, and 4 after the procedure, control, unrepaired, and repaired fetal heads were obtained, sectioned, and stained for histologic examination. The localization of hyaluronic acid in the wound was documented using a cartilage-derived hyaluronic acid-binding protein. In both repaired and unrepaired wounds, the fetal cleft healed without inflammatory cell infiltration or scar formation. Six months after birth, the repaired cleft showed complete regeneration of muscle across the wound and the collagen fibers were of normal density and orientation. Decreased hyaluronic acid deposition was observed in unrepaired clefts as compared with adjacent tissue; no such difference was detected in repaired clefts. Our findings support the hypothesis that a cleft lip repaired in utero heals without the scarring that accompanies postnatal repair. This may explain the lack of maxillary growth restriction after in utero cleft lip repair.

Original languageEnglish (US)
Pages (from-to)263-268
Number of pages6
JournalJournal of Oral and Maxillofacial Surgery
Volume50
Issue number3
DOIs
StatePublished - 1992

Fingerprint

Cleft Lip
Hyaluronic Acid
Histology
Rabbits
Cicatrix
Wounds and Injuries
CD44 Antigens
Third Pregnancy Trimester
Wound Healing
Cartilage
Regeneration
Collagen
Head
Parturition
Muscles
Growth

ASJC Scopus subject areas

  • Surgery
  • Dentistry(all)

Cite this

Fetal cleft lip repair in rabbits : Histology and role of hyaluronic acid. / Stern, Michael; Schmidt, Brian; Dodson, Thomas B.; Stern, Robert; Kaban, Leonard B.

In: Journal of Oral and Maxillofacial Surgery, Vol. 50, No. 3, 1992, p. 263-268.

Research output: Contribution to journalArticle

Stern, Michael ; Schmidt, Brian ; Dodson, Thomas B. ; Stern, Robert ; Kaban, Leonard B. / Fetal cleft lip repair in rabbits : Histology and role of hyaluronic acid. In: Journal of Oral and Maxillofacial Surgery. 1992 ; Vol. 50, No. 3. pp. 263-268.
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