Faeces as a source of DNA for molecular studies in a threatened population of great bustards

Youssef Idaghdhour, D. Broderick, A. Korrida

    Research output: Contribution to journalArticle

    Abstract

    With recent advances in molecular biology, it is now possible to use the trace amounts of DNA in faeces to non-invasively sample endangered species for genetic studies. A highly vulnerable population of approximately 100 great bustards (Otis tarda) exists in Morocco necessitating the use of non-invasive protocols to study their genetic structure. Here we report a reliable silica-based method to extract DNA from great bustard faeces. We found that successful extraction and amplification correlated strongly with faeces freshness and composition. We could not extract amplifiable DNA from 30% of our samples as they were dry or contained insect material. However 100% of our fresh faecal samples containing no obvious insect material worked, allowing us to assess the levels of genetic variation among 25 individuals using a 542 bp control region sequence. We were able to extract DNA from four out of five other avian species, demonstrating that faeces represents a suitable source of DNA for population genetics studies in a broad range of species.

    Original languageEnglish (US)
    Pages (from-to)789-792
    Number of pages4
    JournalConservation Genetics
    Volume4
    Issue number6
    DOIs
    StatePublished - Dec 1 2003

    Fingerprint

    Feces
    feces
    DNA
    Population
    Insects
    extracts
    insect
    Endangered Species
    Morocco
    insects
    Genetic Structures
    freshness
    Population Genetics
    Vulnerable Populations
    endangered species
    sampling
    Silicon Dioxide
    silica
    molecular biology
    genetic structure

    Keywords

    • DNA extraction
    • Faeces
    • mtDNA
    • Non-invasive genetics
    • Otis tarda

    ASJC Scopus subject areas

    • Agricultural and Biological Sciences(all)
    • Genetics
    • Genetics(clinical)
    • Nature and Landscape Conservation

    Cite this

    Faeces as a source of DNA for molecular studies in a threatened population of great bustards. / Idaghdhour, Youssef; Broderick, D.; Korrida, A.

    In: Conservation Genetics, Vol. 4, No. 6, 01.12.2003, p. 789-792.

    Research output: Contribution to journalArticle

    Idaghdhour, Youssef ; Broderick, D. ; Korrida, A. / Faeces as a source of DNA for molecular studies in a threatened population of great bustards. In: Conservation Genetics. 2003 ; Vol. 4, No. 6. pp. 789-792.
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