Faeces as a source of DNA for molecular studies in a threatened population of great bustards

Youssef Idaghdhour, D. Broderick, A. Korrida

Research output: Contribution to journalArticle

Abstract

With recent advances in molecular biology, it is now possible to use the trace amounts of DNA in faeces to non-invasively sample endangered species for genetic studies. A highly vulnerable population of approximately 100 great bustards (Otis tarda) exists in Morocco necessitating the use of non-invasive protocols to study their genetic structure. Here we report a reliable silica-based method to extract DNA from great bustard faeces. We found that successful extraction and amplification correlated strongly with faeces freshness and composition. We could not extract amplifiable DNA from 30% of our samples as they were dry or contained insect material. However 100% of our fresh faecal samples containing no obvious insect material worked, allowing us to assess the levels of genetic variation among 25 individuals using a 542 bp control region sequence. We were able to extract DNA from four out of five other avian species, demonstrating that faeces represents a suitable source of DNA for population genetics studies in a broad range of species.

Original languageEnglish (US)
Pages (from-to)789-792
Number of pages4
JournalConservation Genetics
Volume4
Issue number6
DOIs
StatePublished - Dec 1 2003

Fingerprint

Feces
feces
DNA
Population
Insects
extracts
insect
Endangered Species
Morocco
insects
Genetic Structures
freshness
Population Genetics
Vulnerable Populations
endangered species
sampling
Silicon Dioxide
silica
molecular biology
genetic structure

Keywords

  • DNA extraction
  • Faeces
  • mtDNA
  • Non-invasive genetics
  • Otis tarda

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Genetics
  • Genetics(clinical)
  • Nature and Landscape Conservation

Cite this

Faeces as a source of DNA for molecular studies in a threatened population of great bustards. / Idaghdhour, Youssef; Broderick, D.; Korrida, A.

In: Conservation Genetics, Vol. 4, No. 6, 01.12.2003, p. 789-792.

Research output: Contribution to journalArticle

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