Fabrication of an oriented lectin microarray

Daniel C. Propheter, Ku Lung Hsu, Lara Mahal

Research output: Contribution to journalArticle

Abstract

To tag or not to tag: An oriented lectin microarray utilizing recombinant lectins with the standard prokaryotic expression tag glutathione S-transferase has been reported. The oriented lectin microarray improves the overall sensitivity and limits of detection by site-specific orientation of recombinant lectins. This continual improvement of the lectin microarray technology enables a deeper understanding of the biological function of glycans. (Figure Presented)

Original languageEnglish (US)
JournalChemBioChem
Volume11
Issue number9
DOIs
StatePublished - Jun 14 2010

Fingerprint

Microarrays
Lectins
Fabrication
Glutathione Transferase
Polysaccharides
Limit of Detection
Technology

Keywords

  • Carbohydrates
  • Glycomics
  • GST-tagged proteins
  • Microarrays
  • Recombinant lectin

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry
  • Molecular Medicine
  • Molecular Biology
  • Medicine(all)

Cite this

Fabrication of an oriented lectin microarray. / Propheter, Daniel C.; Hsu, Ku Lung; Mahal, Lara.

In: ChemBioChem, Vol. 11, No. 9, 14.06.2010.

Research output: Contribution to journalArticle

Propheter, Daniel C. ; Hsu, Ku Lung ; Mahal, Lara. / Fabrication of an oriented lectin microarray. In: ChemBioChem. 2010 ; Vol. 11, No. 9.
@article{f77179b0aa4849aa9ad3a6d3d45fb87a,
title = "Fabrication of an oriented lectin microarray",
abstract = "To tag or not to tag: An oriented lectin microarray utilizing recombinant lectins with the standard prokaryotic expression tag glutathione S-transferase has been reported. The oriented lectin microarray improves the overall sensitivity and limits of detection by site-specific orientation of recombinant lectins. This continual improvement of the lectin microarray technology enables a deeper understanding of the biological function of glycans. (Figure Presented)",
keywords = "Carbohydrates, Glycomics, GST-tagged proteins, Microarrays, Recombinant lectin",
author = "Propheter, {Daniel C.} and Hsu, {Ku Lung} and Lara Mahal",
year = "2010",
month = "6",
day = "14",
doi = "10.1002/cbic.201000106",
language = "English (US)",
volume = "11",
journal = "ChemBioChem",
issn = "1439-4227",
publisher = "Wiley-VCH Verlag",
number = "9",

}

TY - JOUR

T1 - Fabrication of an oriented lectin microarray

AU - Propheter, Daniel C.

AU - Hsu, Ku Lung

AU - Mahal, Lara

PY - 2010/6/14

Y1 - 2010/6/14

N2 - To tag or not to tag: An oriented lectin microarray utilizing recombinant lectins with the standard prokaryotic expression tag glutathione S-transferase has been reported. The oriented lectin microarray improves the overall sensitivity and limits of detection by site-specific orientation of recombinant lectins. This continual improvement of the lectin microarray technology enables a deeper understanding of the biological function of glycans. (Figure Presented)

AB - To tag or not to tag: An oriented lectin microarray utilizing recombinant lectins with the standard prokaryotic expression tag glutathione S-transferase has been reported. The oriented lectin microarray improves the overall sensitivity and limits of detection by site-specific orientation of recombinant lectins. This continual improvement of the lectin microarray technology enables a deeper understanding of the biological function of glycans. (Figure Presented)

KW - Carbohydrates

KW - Glycomics

KW - GST-tagged proteins

KW - Microarrays

KW - Recombinant lectin

UR - http://www.scopus.com/inward/record.url?scp=77953420571&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77953420571&partnerID=8YFLogxK

U2 - 10.1002/cbic.201000106

DO - 10.1002/cbic.201000106

M3 - Article

VL - 11

JO - ChemBioChem

JF - ChemBioChem

SN - 1439-4227

IS - 9

ER -