FAAH genetic variation enhances fronto-amygdala function in mouse and human

Iva Dincheva, Andrew T. Drysdale, Catherine A. Hartley, David C. Johnson, Deqiang Jing, Elizabeth C. King, Stephen Ra, J. Megan Gray, Ruirong Yang, Ann Marie DeGruccio, Chienchun Huang, Benjamin F. Cravatt, Charles E. Glatt, Matthew N. Hill, B. J. Casey, Francis S. Lee

Research output: Contribution to journalArticle

Abstract

Cross-species studies enable rapid translational discovery and produce the broadest impact when both mechanism and phenotype are consistent across organisms. We developed a knock-in mouse that biologically recapitulates a common human mutation in the gene for fatty acid amide hydrolase (FAAH) (C385A; rs324420), the primary catabolic enzyme for the endocannabinoid anandamide. This common polymorphism impacts the expression and activity of FAAH, thereby increasing anandamide levels. Here, we show that the genetic knock-in mouse and human variant allele carriers exhibit parallel alterations in biochemisty, neurocircuitry and behaviour. Specifically, there is reduced FAAH expression associated with the variant allele that selectively enhances fronto-amygdala connectivity and fear extinction learning, and decreases anxiety-like behaviours. These results suggest a gain of function in fear regulation and may indicate for whom and for what anxiety symptoms FAAH inhibitors or exposure-based therapies will be most efficacious, bridging an important translational gap between the mouse and human.

Original languageEnglish (US)
Article number6395
JournalNature communications
Volume6
DOIs
StatePublished - 2015

    Fingerprint

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Dincheva, I., Drysdale, A. T., Hartley, C. A., Johnson, D. C., Jing, D., King, E. C., Ra, S., Gray, J. M., Yang, R., DeGruccio, A. M., Huang, C., Cravatt, B. F., Glatt, C. E., Hill, M. N., Casey, B. J., & Lee, F. S. (2015). FAAH genetic variation enhances fronto-amygdala function in mouse and human. Nature communications, 6, [6395]. https://doi.org/10.1038/ncomms7395