Expression of the sodium/calcium/potassium exchanger, NCKX4, in Ameloblasts

Ping Hu, Rodrigo Lacruz, Charles E. Smith, Susan M. Smith, Ira Kurtz, Michael L. Paine

Research output: Contribution to journalArticle

Abstract

Transcellular calcium transport is an essential activity in mineralized tissue formation, including dental hard tissues. In many organ systems, this activity is regulated by membrane-bound sodium/calcium (Na+/Ca 2+) exchangers, which include the NCX and NCKX [sodium/calcium- potassium (Na+/Ca2+-K+) exchanger] proteins. During enamel maturation, when crystals expand in thickness, Ca2+ requirements vastly increase but exactly how Ca2+ traffics through ameloblasts remains uncertain. Previous studies have shown that several NCX proteins are expressed in ameloblasts, although no significant shifts in expression were observed during maturation which pointed to the possible identification of other Ca2+ membrane transporters. NCKX proteins are encoded by members of the solute carrier gene family, Slc24a, which include 6 different proteins (NCKX1-6). NCKX are bidirectional electrogenic transporters regulating Ca2+ transport in and out of cells dependent on the transmembrane ion gradient. In this study we show that all NCKX mRNAs are expressed in dental tissues. Real-time PCR indicates that of all the members of the NCKX group, NCKX4 is the most highly expressed gene transcript during the late stages of amelogenesis. In situ hybridization and immunolocalization analyses clearly establish that in the enamel organ, NCKX4 is expressed primarily by ameloblasts during the maturation stage. Further, during the mid-late maturation stages of amelogenesis, the expression of NCKX4 in ameloblasts is most prominent at the apical poles and at the lateral membranes proximal to the apical ends. These data suggest that NCKX4 might be an important regulator of Ca2+ transport during amelogenesis.

Original languageEnglish (US)
Pages (from-to)501-509
Number of pages9
JournalCells Tissues Organs
Volume196
Issue number6
DOIs
StatePublished - Nov 2012

Fingerprint

Ameloblasts
Amelogenesis
Calcium
Tooth
Enamel Organ
Sodium
Sodium-Calcium Exchanger
Transcytosis
Proteins
Membranes
Membrane Transport Proteins
Dental Enamel
Genes
In Situ Hybridization
Real-Time Polymerase Chain Reaction
Potassium
Ions
Messenger RNA
potassium-dependent sodium-calcium exchanger

Keywords

  • Amelogenesis
  • Enamel
  • Mouse
  • Sodium/calcium exchanger
  • Sodium/calcium/potassium exchanger
  • Solute carrier gene

ASJC Scopus subject areas

  • Anatomy
  • Histology

Cite this

Expression of the sodium/calcium/potassium exchanger, NCKX4, in Ameloblasts. / Hu, Ping; Lacruz, Rodrigo; Smith, Charles E.; Smith, Susan M.; Kurtz, Ira; Paine, Michael L.

In: Cells Tissues Organs, Vol. 196, No. 6, 11.2012, p. 501-509.

Research output: Contribution to journalArticle

Hu, Ping ; Lacruz, Rodrigo ; Smith, Charles E. ; Smith, Susan M. ; Kurtz, Ira ; Paine, Michael L. / Expression of the sodium/calcium/potassium exchanger, NCKX4, in Ameloblasts. In: Cells Tissues Organs. 2012 ; Vol. 196, No. 6. pp. 501-509.
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