Expression of different glycosaminoglycan synthetic phenotypes by lapine dermal and dermal wound fibroblasts

Charles Bertolami, R. E. Bronson

Research output: Contribution to journalArticle

Abstract

Synthesis of extracellular matrix by dermal fibroblasts is an important component of cutaneous wound repair. Scar remodeling and maturation is generally seen as the result of a fibroblast-regulated equilibrium between production and degradation of specific matrix constituents. Fibroblasts from normal dermis, reparative granulation tissue and mature scars were compared in vitro in terms of their ability to produce extracellular glycosaminoglycans (GAGs). All cell lines secreted dermatan sulfate (DS) and chondroitin sulfate (CS) into the culture medium. Hyaluronate (HA) was detected in medium from mature granulation tissue and scar cells, but little or none was found in medium from early granulation tissue or skin cells. In medium from normal skin fibroblasts, an unusual GAG was identified as a potential variant of DS on the basis of co-migration with HA but susceptibility to digestion with chondroitinase ABC. Heparan sulfate (HS) was the major pericellular GAG of all cultures except the mature scar cells, which contained a predominance of DS. A second pericellular GAG was identified as CS in mature granulation tissue cells, scar cells and skin cells; while HA was identified in the pericellular matrix of early granulation tissue cells. In addition, fibroblasts from both skin and early granulation tissue contained a GAG believed to be a variant of CS. These differences in GAG synthesis/secretion between cells maintained under identical culturing conditions could indicate either that distinct fibroblastic substrains exist during different stages of healing or that influences present during the healing process induce stable phenotypic alterations that are maintained through explant culturing and subsequent subcultivation.

Original languageEnglish (US)
Pages (from-to)1-9
Number of pages9
JournalMatrix
Volume10
Issue number1
StatePublished - 1990

Fingerprint

Glycosaminoglycans
Granulation Tissue
Fibroblasts
Phenotype
Skin
Cicatrix
Wounds and Injuries
Dermatan Sulfate
Chondroitin Sulfates
Chondroitin ABC Lyase
Heparitin Sulfate
Dermis
Extracellular Matrix
Culture Media
Digestion
Cell Line

Keywords

  • Fibroblasts
  • Glycosaminoglycans
  • Scar
  • Skin
  • Wound healing

ASJC Scopus subject areas

  • Rheumatology

Cite this

Expression of different glycosaminoglycan synthetic phenotypes by lapine dermal and dermal wound fibroblasts. / Bertolami, Charles; Bronson, R. E.

In: Matrix, Vol. 10, No. 1, 1990, p. 1-9.

Research output: Contribution to journalArticle

@article{d5b960851e374685ab0826e2ec14bff5,
title = "Expression of different glycosaminoglycan synthetic phenotypes by lapine dermal and dermal wound fibroblasts",
abstract = "Synthesis of extracellular matrix by dermal fibroblasts is an important component of cutaneous wound repair. Scar remodeling and maturation is generally seen as the result of a fibroblast-regulated equilibrium between production and degradation of specific matrix constituents. Fibroblasts from normal dermis, reparative granulation tissue and mature scars were compared in vitro in terms of their ability to produce extracellular glycosaminoglycans (GAGs). All cell lines secreted dermatan sulfate (DS) and chondroitin sulfate (CS) into the culture medium. Hyaluronate (HA) was detected in medium from mature granulation tissue and scar cells, but little or none was found in medium from early granulation tissue or skin cells. In medium from normal skin fibroblasts, an unusual GAG was identified as a potential variant of DS on the basis of co-migration with HA but susceptibility to digestion with chondroitinase ABC. Heparan sulfate (HS) was the major pericellular GAG of all cultures except the mature scar cells, which contained a predominance of DS. A second pericellular GAG was identified as CS in mature granulation tissue cells, scar cells and skin cells; while HA was identified in the pericellular matrix of early granulation tissue cells. In addition, fibroblasts from both skin and early granulation tissue contained a GAG believed to be a variant of CS. These differences in GAG synthesis/secretion between cells maintained under identical culturing conditions could indicate either that distinct fibroblastic substrains exist during different stages of healing or that influences present during the healing process induce stable phenotypic alterations that are maintained through explant culturing and subsequent subcultivation.",
keywords = "Fibroblasts, Glycosaminoglycans, Scar, Skin, Wound healing",
author = "Charles Bertolami and Bronson, {R. E.}",
year = "1990",
language = "English (US)",
volume = "10",
pages = "1--9",
journal = "Matrix Biology",
issn = "0945-053X",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Expression of different glycosaminoglycan synthetic phenotypes by lapine dermal and dermal wound fibroblasts

AU - Bertolami, Charles

AU - Bronson, R. E.

PY - 1990

Y1 - 1990

N2 - Synthesis of extracellular matrix by dermal fibroblasts is an important component of cutaneous wound repair. Scar remodeling and maturation is generally seen as the result of a fibroblast-regulated equilibrium between production and degradation of specific matrix constituents. Fibroblasts from normal dermis, reparative granulation tissue and mature scars were compared in vitro in terms of their ability to produce extracellular glycosaminoglycans (GAGs). All cell lines secreted dermatan sulfate (DS) and chondroitin sulfate (CS) into the culture medium. Hyaluronate (HA) was detected in medium from mature granulation tissue and scar cells, but little or none was found in medium from early granulation tissue or skin cells. In medium from normal skin fibroblasts, an unusual GAG was identified as a potential variant of DS on the basis of co-migration with HA but susceptibility to digestion with chondroitinase ABC. Heparan sulfate (HS) was the major pericellular GAG of all cultures except the mature scar cells, which contained a predominance of DS. A second pericellular GAG was identified as CS in mature granulation tissue cells, scar cells and skin cells; while HA was identified in the pericellular matrix of early granulation tissue cells. In addition, fibroblasts from both skin and early granulation tissue contained a GAG believed to be a variant of CS. These differences in GAG synthesis/secretion between cells maintained under identical culturing conditions could indicate either that distinct fibroblastic substrains exist during different stages of healing or that influences present during the healing process induce stable phenotypic alterations that are maintained through explant culturing and subsequent subcultivation.

AB - Synthesis of extracellular matrix by dermal fibroblasts is an important component of cutaneous wound repair. Scar remodeling and maturation is generally seen as the result of a fibroblast-regulated equilibrium between production and degradation of specific matrix constituents. Fibroblasts from normal dermis, reparative granulation tissue and mature scars were compared in vitro in terms of their ability to produce extracellular glycosaminoglycans (GAGs). All cell lines secreted dermatan sulfate (DS) and chondroitin sulfate (CS) into the culture medium. Hyaluronate (HA) was detected in medium from mature granulation tissue and scar cells, but little or none was found in medium from early granulation tissue or skin cells. In medium from normal skin fibroblasts, an unusual GAG was identified as a potential variant of DS on the basis of co-migration with HA but susceptibility to digestion with chondroitinase ABC. Heparan sulfate (HS) was the major pericellular GAG of all cultures except the mature scar cells, which contained a predominance of DS. A second pericellular GAG was identified as CS in mature granulation tissue cells, scar cells and skin cells; while HA was identified in the pericellular matrix of early granulation tissue cells. In addition, fibroblasts from both skin and early granulation tissue contained a GAG believed to be a variant of CS. These differences in GAG synthesis/secretion between cells maintained under identical culturing conditions could indicate either that distinct fibroblastic substrains exist during different stages of healing or that influences present during the healing process induce stable phenotypic alterations that are maintained through explant culturing and subsequent subcultivation.

KW - Fibroblasts

KW - Glycosaminoglycans

KW - Scar

KW - Skin

KW - Wound healing

UR - http://www.scopus.com/inward/record.url?scp=0025259392&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025259392&partnerID=8YFLogxK

M3 - Article

VL - 10

SP - 1

EP - 9

JO - Matrix Biology

JF - Matrix Biology

SN - 0945-053X

IS - 1

ER -