Expression of collagenase and IL-1α in developing rat hearts

M. Nakagawa, Louis Terracio, W. Carver, H. Birkedal-Hansen, T. K. Borg

Research output: Contribution to journalArticle

Abstract

During development, extracellular matrix (ECM) molecules are thought to play a major role in regulating the formation of the heart. The change in the heart from a simple tube to a complex, four-chambered organ requires the modification of both the cellular components as well as the surrounding ECM. Matrix metalloproteinases (MMP), which include collagenases, are enzymes present in the ECM that have the potential to modify the existing ECM during the development of the heart. Using both monoclonal and polyclonal antisera against collagenase, specific temporal and spatial patterns have been documented during critical periods of heart development. The cytokine interleukin 1α (IL-1α), a potent inducer of the MMP expression, was also shown to have a similar staining pattern in the developing heart. The monoclonal anti-rat collagenase (Mab) intensely stained the surfaces of the myocytes in the trabeculae and the ventricular and atrial walls of the 11.5 or 12.5 embryonic day (ED) rat hearts. In contrast, the polyclonal anti- human collagenase (Pab) stained not only the cardiomyocytes but also the hypertrophic endocardial cells. Pab appeared to stain the leading edge of the mesenchymal cells that migrate into the cardiac jelly of the 11.5 or 12.5 ED hearts. Immunohistochemical staining showed IL-1α on the endocardial endothelium and the surface of cardiomyocytes near the cardiac jelly just before or coincident with the appearance of migrating cells. IL-1α was detected on the endocardial endothelium, cardiomyocytes in the trabeculae, and the ventricular and atrial walls, as well as in the myocardial basement membrane of the truncal or atrioventricular region. However, no staining could be detected on the migrating cells in the cardiac cushions. These results indicate the presence of collagenase and IL-1α on the surface of cardiomyocytes and mesenchymal cells at times when the heart is undergoing acute remodeling during septation and trabeculation. These data suggest a role for collagenase/cytokine interaction in tissue remodeling during critical stages of cardiac embryogenesis where modification of the ECM is essential to cardiac morphogenesis.

Original languageEnglish (US)
Pages (from-to)87-99
Number of pages13
JournalDevelopmental Dynamics
Volume195
Issue number2
StatePublished - 1992

Fingerprint

Interleukin-1
Collagenases
Extracellular Matrix
Cardiac Myocytes
Staining and Labeling
Matrix Metalloproteinases
Endothelium
Cytokines
collagenase 1
Morphogenesis
Basement Membrane
Muscle Cells
Embryonic Development
Immune Sera
Coloring Agents
Enzymes

Keywords

  • Extracellular matrix
  • Matrix mettaloproteinases
  • Monoclonal anti-rat collagenase
  • Polyclonal anti-human collagenase
  • Septation
  • Trabeculation

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology

Cite this

Nakagawa, M., Terracio, L., Carver, W., Birkedal-Hansen, H., & Borg, T. K. (1992). Expression of collagenase and IL-1α in developing rat hearts. Developmental Dynamics, 195(2), 87-99.

Expression of collagenase and IL-1α in developing rat hearts. / Nakagawa, M.; Terracio, Louis; Carver, W.; Birkedal-Hansen, H.; Borg, T. K.

In: Developmental Dynamics, Vol. 195, No. 2, 1992, p. 87-99.

Research output: Contribution to journalArticle

Nakagawa, M, Terracio, L, Carver, W, Birkedal-Hansen, H & Borg, TK 1992, 'Expression of collagenase and IL-1α in developing rat hearts', Developmental Dynamics, vol. 195, no. 2, pp. 87-99.
Nakagawa M, Terracio L, Carver W, Birkedal-Hansen H, Borg TK. Expression of collagenase and IL-1α in developing rat hearts. Developmental Dynamics. 1992;195(2):87-99.
Nakagawa, M. ; Terracio, Louis ; Carver, W. ; Birkedal-Hansen, H. ; Borg, T. K. / Expression of collagenase and IL-1α in developing rat hearts. In: Developmental Dynamics. 1992 ; Vol. 195, No. 2. pp. 87-99.
@article{940fe93e878541459572b0e70fb696fe,
title = "Expression of collagenase and IL-1α in developing rat hearts",
abstract = "During development, extracellular matrix (ECM) molecules are thought to play a major role in regulating the formation of the heart. The change in the heart from a simple tube to a complex, four-chambered organ requires the modification of both the cellular components as well as the surrounding ECM. Matrix metalloproteinases (MMP), which include collagenases, are enzymes present in the ECM that have the potential to modify the existing ECM during the development of the heart. Using both monoclonal and polyclonal antisera against collagenase, specific temporal and spatial patterns have been documented during critical periods of heart development. The cytokine interleukin 1α (IL-1α), a potent inducer of the MMP expression, was also shown to have a similar staining pattern in the developing heart. The monoclonal anti-rat collagenase (Mab) intensely stained the surfaces of the myocytes in the trabeculae and the ventricular and atrial walls of the 11.5 or 12.5 embryonic day (ED) rat hearts. In contrast, the polyclonal anti- human collagenase (Pab) stained not only the cardiomyocytes but also the hypertrophic endocardial cells. Pab appeared to stain the leading edge of the mesenchymal cells that migrate into the cardiac jelly of the 11.5 or 12.5 ED hearts. Immunohistochemical staining showed IL-1α on the endocardial endothelium and the surface of cardiomyocytes near the cardiac jelly just before or coincident with the appearance of migrating cells. IL-1α was detected on the endocardial endothelium, cardiomyocytes in the trabeculae, and the ventricular and atrial walls, as well as in the myocardial basement membrane of the truncal or atrioventricular region. However, no staining could be detected on the migrating cells in the cardiac cushions. These results indicate the presence of collagenase and IL-1α on the surface of cardiomyocytes and mesenchymal cells at times when the heart is undergoing acute remodeling during septation and trabeculation. These data suggest a role for collagenase/cytokine interaction in tissue remodeling during critical stages of cardiac embryogenesis where modification of the ECM is essential to cardiac morphogenesis.",
keywords = "Extracellular matrix, Matrix mettaloproteinases, Monoclonal anti-rat collagenase, Polyclonal anti-human collagenase, Septation, Trabeculation",
author = "M. Nakagawa and Louis Terracio and W. Carver and H. Birkedal-Hansen and Borg, {T. K.}",
year = "1992",
language = "English (US)",
volume = "195",
pages = "87--99",
journal = "Developmental Dynamics",
issn = "1058-8388",
publisher = "Wiley-Liss Inc.",
number = "2",

}

TY - JOUR

T1 - Expression of collagenase and IL-1α in developing rat hearts

AU - Nakagawa, M.

AU - Terracio, Louis

AU - Carver, W.

AU - Birkedal-Hansen, H.

AU - Borg, T. K.

PY - 1992

Y1 - 1992

N2 - During development, extracellular matrix (ECM) molecules are thought to play a major role in regulating the formation of the heart. The change in the heart from a simple tube to a complex, four-chambered organ requires the modification of both the cellular components as well as the surrounding ECM. Matrix metalloproteinases (MMP), which include collagenases, are enzymes present in the ECM that have the potential to modify the existing ECM during the development of the heart. Using both monoclonal and polyclonal antisera against collagenase, specific temporal and spatial patterns have been documented during critical periods of heart development. The cytokine interleukin 1α (IL-1α), a potent inducer of the MMP expression, was also shown to have a similar staining pattern in the developing heart. The monoclonal anti-rat collagenase (Mab) intensely stained the surfaces of the myocytes in the trabeculae and the ventricular and atrial walls of the 11.5 or 12.5 embryonic day (ED) rat hearts. In contrast, the polyclonal anti- human collagenase (Pab) stained not only the cardiomyocytes but also the hypertrophic endocardial cells. Pab appeared to stain the leading edge of the mesenchymal cells that migrate into the cardiac jelly of the 11.5 or 12.5 ED hearts. Immunohistochemical staining showed IL-1α on the endocardial endothelium and the surface of cardiomyocytes near the cardiac jelly just before or coincident with the appearance of migrating cells. IL-1α was detected on the endocardial endothelium, cardiomyocytes in the trabeculae, and the ventricular and atrial walls, as well as in the myocardial basement membrane of the truncal or atrioventricular region. However, no staining could be detected on the migrating cells in the cardiac cushions. These results indicate the presence of collagenase and IL-1α on the surface of cardiomyocytes and mesenchymal cells at times when the heart is undergoing acute remodeling during septation and trabeculation. These data suggest a role for collagenase/cytokine interaction in tissue remodeling during critical stages of cardiac embryogenesis where modification of the ECM is essential to cardiac morphogenesis.

AB - During development, extracellular matrix (ECM) molecules are thought to play a major role in regulating the formation of the heart. The change in the heart from a simple tube to a complex, four-chambered organ requires the modification of both the cellular components as well as the surrounding ECM. Matrix metalloproteinases (MMP), which include collagenases, are enzymes present in the ECM that have the potential to modify the existing ECM during the development of the heart. Using both monoclonal and polyclonal antisera against collagenase, specific temporal and spatial patterns have been documented during critical periods of heart development. The cytokine interleukin 1α (IL-1α), a potent inducer of the MMP expression, was also shown to have a similar staining pattern in the developing heart. The monoclonal anti-rat collagenase (Mab) intensely stained the surfaces of the myocytes in the trabeculae and the ventricular and atrial walls of the 11.5 or 12.5 embryonic day (ED) rat hearts. In contrast, the polyclonal anti- human collagenase (Pab) stained not only the cardiomyocytes but also the hypertrophic endocardial cells. Pab appeared to stain the leading edge of the mesenchymal cells that migrate into the cardiac jelly of the 11.5 or 12.5 ED hearts. Immunohistochemical staining showed IL-1α on the endocardial endothelium and the surface of cardiomyocytes near the cardiac jelly just before or coincident with the appearance of migrating cells. IL-1α was detected on the endocardial endothelium, cardiomyocytes in the trabeculae, and the ventricular and atrial walls, as well as in the myocardial basement membrane of the truncal or atrioventricular region. However, no staining could be detected on the migrating cells in the cardiac cushions. These results indicate the presence of collagenase and IL-1α on the surface of cardiomyocytes and mesenchymal cells at times when the heart is undergoing acute remodeling during septation and trabeculation. These data suggest a role for collagenase/cytokine interaction in tissue remodeling during critical stages of cardiac embryogenesis where modification of the ECM is essential to cardiac morphogenesis.

KW - Extracellular matrix

KW - Matrix mettaloproteinases

KW - Monoclonal anti-rat collagenase

KW - Polyclonal anti-human collagenase

KW - Septation

KW - Trabeculation

UR - http://www.scopus.com/inward/record.url?scp=0027083150&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027083150&partnerID=8YFLogxK

M3 - Article

C2 - 1297459

AN - SCOPUS:0027083150

VL - 195

SP - 87

EP - 99

JO - Developmental Dynamics

JF - Developmental Dynamics

SN - 1058-8388

IS - 2

ER -