Expression of collagen binding integrins during cardiac development and hypertrophy

Louis Terracio, K. Rubin, D. Gullberg, E. Balog, W. Carver, R. Jyring, T. K. Borg

Research output: Contribution to journalArticle

Abstract

The interaction between components of the extracellular matrix and the cell surface of cardiac myocytes appears to be regulated in part by receptors belonging to the integrin superfamily. The expression of the integrins was investigated at different stages of development of the heart as well as during cardiac hypertrophy. The characterization of the membrane proteins showed that a β1-integrin and associated α-chains were responsible for the interaction with collagen, laminin, and fibronectin. Immunoprecipitation data indicated that the presence of specific α-chains varied with development. These data were correlated with the ability of the isolated myocytes to attach to specific components of the extracellular matrix. The expression of the α1-chain was prominently associated with the recognition of interstitial collagens. The presence of the α1-chain was also associated with stages when collagen synthesis was increased, especially during letal and neonatal growth and cardiac hypertrophy. Immunohistochemical localization with the antiserum against β1-integrin demonstrated its specific localization near the Z lines of cardiac myocytes. The localization both in vitro and in vivo indicated that the β1-integrin may play a role in myofibrillogenesis during development. The present immunohistochemical, cell adhesion, and biochemical data clearly indicate that integrins play a major role in the regulation of the interaction between cardiac myocytes and the extracellular matrix during development and disease.

Original languageEnglish (US)
Pages (from-to)734-744
Number of pages11
JournalCirculation Research
Volume68
Issue number3
StatePublished - 1991

Fingerprint

Cardiomegaly
Integrins
Collagen
Cardiac Myocytes
Extracellular Matrix
Muscle Development
Laminin
Fibronectins
Immunoprecipitation
Cell Adhesion
Muscle Cells
Immune Sera
Membrane Proteins
Growth

Keywords

  • cardiac development
  • cardiac hypertrophy
  • collagen binding
  • extracellular matrix
  • integrins

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Terracio, L., Rubin, K., Gullberg, D., Balog, E., Carver, W., Jyring, R., & Borg, T. K. (1991). Expression of collagen binding integrins during cardiac development and hypertrophy. Circulation Research, 68(3), 734-744.

Expression of collagen binding integrins during cardiac development and hypertrophy. / Terracio, Louis; Rubin, K.; Gullberg, D.; Balog, E.; Carver, W.; Jyring, R.; Borg, T. K.

In: Circulation Research, Vol. 68, No. 3, 1991, p. 734-744.

Research output: Contribution to journalArticle

Terracio, L, Rubin, K, Gullberg, D, Balog, E, Carver, W, Jyring, R & Borg, TK 1991, 'Expression of collagen binding integrins during cardiac development and hypertrophy', Circulation Research, vol. 68, no. 3, pp. 734-744.
Terracio L, Rubin K, Gullberg D, Balog E, Carver W, Jyring R et al. Expression of collagen binding integrins during cardiac development and hypertrophy. Circulation Research. 1991;68(3):734-744.
Terracio, Louis ; Rubin, K. ; Gullberg, D. ; Balog, E. ; Carver, W. ; Jyring, R. ; Borg, T. K. / Expression of collagen binding integrins during cardiac development and hypertrophy. In: Circulation Research. 1991 ; Vol. 68, No. 3. pp. 734-744.
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