Expression of apoptosis-associated genes by human dermal scar fibroblasts

Diana V. Messadi, Anh Le, Steve Berg, Anahid Jewett, Zhuang Wen, Paul Kelly, Charles Bertolami

Research output: Contribution to journalArticle

Abstract

The purpose of this study was to determine if aberrant apoptosis plays a role in pathologic wound healing as manifested by hypertrophic scarring and keloid formation. Apoptosis has recently been found to participate in the transition between granulation tissue and the development of definitive scar. The question that remains to be answered is what stimuli initiate apoptosis during wound healing. Hitherto, regulatory factors and pathways involved have been largely undefined. We investigated heterogeneity among fibroblasts derived from normal skin and keloid scar, by examining apoptotic profiles and pathways for these cells. Quantitative analysis of apoptotic cells using an Annexin-V-FITC binding assay showed that normal skin fibroblast cultures were found to have a two-fold higher percentage of apoptotic cells than did keloid fibroblast cultures. To study apoptotic pathways and related death-associated genes, a ribonuclease protection assay was performed for fibroblasts exposed to anti-Fas antibody and tumor necrosis factor-α to activate the Fas/TNF receptor apoptotic pathway. Compared with normal skin fibroblasts, keloid fibroblasts exhibited decreased expression of apoptosis-associated genes.

Original languageEnglish (US)
Pages (from-to)511-517
Number of pages7
JournalWound Repair and Regeneration
Volume7
Issue number6
DOIs
StatePublished - 1999

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Cicatrix
Keloid
Fibroblasts
Apoptosis
Skin
Genes
Wound Healing
CD95 Antigens
Granulation Tissue
Fluorescein-5-isothiocyanate
Tumor Necrosis Factor Receptors
Annexin A5
Ribonucleases
Anti-Idiotypic Antibodies
Tumor Necrosis Factor-alpha

ASJC Scopus subject areas

  • Dermatology
  • Surgery

Cite this

Expression of apoptosis-associated genes by human dermal scar fibroblasts. / Messadi, Diana V.; Le, Anh; Berg, Steve; Jewett, Anahid; Wen, Zhuang; Kelly, Paul; Bertolami, Charles.

In: Wound Repair and Regeneration, Vol. 7, No. 6, 1999, p. 511-517.

Research output: Contribution to journalArticle

Messadi, Diana V. ; Le, Anh ; Berg, Steve ; Jewett, Anahid ; Wen, Zhuang ; Kelly, Paul ; Bertolami, Charles. / Expression of apoptosis-associated genes by human dermal scar fibroblasts. In: Wound Repair and Regeneration. 1999 ; Vol. 7, No. 6. pp. 511-517.
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