Expression analysis of Runx3 and other Runx family members during Xenopus development

Byung Yong Park, Jean-Pierre Saint-Jeannet

Research output: Contribution to journalArticle

Abstract

Runx genes encode a family of proteins defined by the highly conserved runt DNA-binding domain. Studies in several organisms have shown that these transcription factors regulate multiple aspects of embryonic development and are responsible for the pathogenesis of several human diseases. Here we report the cloning and expression of Runx3 during Xenopus development and compare its expression pattern to other Runx family members, Runx1 and Runx2, and to Cbfb, the obligatory binding partner of Runx proteins. Using in situ hybridization in the whole embryo and on sections we show that Runx3 is coexpressed with Runx1 in the hematopoietic lineage and in Rohon-Beard sensory neurons. In contrast Runx3 and Runx2 are co-expressed in craniofacial cartilage elements. Runx3 shows also unique expression domains in a number of derivatives of the neurogenic placodes, including the ganglia of the anteroposterior and middle lateral line nerves, and ganglia of the trigeminal, glossopharyngeal, facial and vagal nerves. These observations suggest a critical role for Runx3 in the development of cranial sensory neurons, while in other tissues its co-expression with Runx1 or Runx2 may signify functional redundancy between these family members.

Original languageEnglish (US)
Pages (from-to)159-166
Number of pages8
JournalGene Expression Patterns
Volume10
Issue number4-5
DOIs
StatePublished - Jun 2010

Fingerprint

Sensory Receptor Cells
Xenopus
Core Binding Factor alpha Subunits
Trigeminal Ganglion
Trigeminal Nerve
Facial Nerve
Ganglia
Cartilage
Embryonic Development
In Situ Hybridization
Organism Cloning
Transcription Factors
Embryonic Structures
DNA
Genes
Proteins

Keywords

  • Cartilage
  • Cbfb
  • Cranial nerve
  • Craniofacial
  • Glossopharyngeal
  • Islet1
  • Lateral line
  • Pax3
  • Placode
  • Profundal
  • Rohon-Beard neuron
  • Runx1
  • Runx2
  • Runx3
  • Trigeminal
  • Vagal
  • Xenopus

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Developmental Biology

Cite this

Expression analysis of Runx3 and other Runx family members during Xenopus development. / Park, Byung Yong; Saint-Jeannet, Jean-Pierre.

In: Gene Expression Patterns, Vol. 10, No. 4-5, 06.2010, p. 159-166.

Research output: Contribution to journalArticle

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