Expressed sequence tag analysis in Cycas, the most primitive living seed plant.

Eric D. Brenner, Dennis W. Stevenson, Richard W. McCombie, Manpreet S. Katari, Stephen A. Rudd, Klaus F X Mayer, Peter M. Palenchar, Suzan J. Runko, Richard W. Twigg, Guangwei Dai, Rob A. Martienssen, Phillip N. Benfey, Gloria Coruzzi

Research output: Contribution to journalArticle

Abstract

BACKGROUND: Cycads are ancient seed plants (living fossils) with origins in the Paleozoic. Cycads are sometimes considered a 'missing link' as they exhibit characteristics intermediate between vascular non-seed plants and the more derived seed plants. Cycads have also been implicated as the source of 'Guam's dementia', possibly due to the production of S(+)-beta-methyl-alpha, beta-diaminopropionic acid (BMAA), which is an agonist of animal glutamate receptors. RESULTS: A total of 4,200 expressed sequence tags (ESTs) were created from Cycas rumphii and clustered into 2,458 contigs, of which 1,764 had low-stringency BLAST similarity to other plant genes. Among those cycad contigs with similarity to plant genes, 1,718 cycad 'hits' are to angiosperms, 1,310 match genes in gymnosperms and 734 match lower (non-seed) plants. Forty-six contigs were found that matched only genes in lower plants and gymnosperms. Upon obtaining the complete sequence from the clones of 37/46 contigs, 14 still matched only gymnosperms. Among those cycad contigs common to higher plants, ESTs were discovered that correspond to those involved in development and signaling in present-day flowering plants. We purified a cycad EST for a glutamate receptor (GLR)-like gene, as well as ESTs potentially involved in the synthesis of the GLR agonist BMAA. CONCLUSIONS: Analysis of cycad ESTs has uncovered conserved and potentially novel genes. Furthermore, the presence of a glutamate receptor agonist, as well as a glutamate receptor-like gene in cycads, supports the hypothesis that such neuroactive plant products are not merely herbivore deterrents but may also serve a role in plant signaling.

Original languageEnglish (US)
JournalGenome Biology
Volume4
Issue number12
StatePublished - 2003

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Cycas
Cycadopsida
Expressed Sequence Tags
Spermatophytina
expressed sequence tags
Sequence Analysis
Seeds
seed
Glutamate Receptors
Gymnosperms
gene
gymnosperm
genes
Excitatory Amino Acid Agonists
Plant Genes
beta acids
Genes
angiosperm
agonists
Angiospermae

ASJC Scopus subject areas

  • Genetics

Cite this

Brenner, E. D., Stevenson, D. W., McCombie, R. W., Katari, M. S., Rudd, S. A., Mayer, K. F. X., ... Coruzzi, G. (2003). Expressed sequence tag analysis in Cycas, the most primitive living seed plant. Genome Biology, 4(12).

Expressed sequence tag analysis in Cycas, the most primitive living seed plant. / Brenner, Eric D.; Stevenson, Dennis W.; McCombie, Richard W.; Katari, Manpreet S.; Rudd, Stephen A.; Mayer, Klaus F X; Palenchar, Peter M.; Runko, Suzan J.; Twigg, Richard W.; Dai, Guangwei; Martienssen, Rob A.; Benfey, Phillip N.; Coruzzi, Gloria.

In: Genome Biology, Vol. 4, No. 12, 2003.

Research output: Contribution to journalArticle

Brenner, ED, Stevenson, DW, McCombie, RW, Katari, MS, Rudd, SA, Mayer, KFX, Palenchar, PM, Runko, SJ, Twigg, RW, Dai, G, Martienssen, RA, Benfey, PN & Coruzzi, G 2003, 'Expressed sequence tag analysis in Cycas, the most primitive living seed plant.', Genome Biology, vol. 4, no. 12.
Brenner ED, Stevenson DW, McCombie RW, Katari MS, Rudd SA, Mayer KFX et al. Expressed sequence tag analysis in Cycas, the most primitive living seed plant. Genome Biology. 2003;4(12).
Brenner, Eric D. ; Stevenson, Dennis W. ; McCombie, Richard W. ; Katari, Manpreet S. ; Rudd, Stephen A. ; Mayer, Klaus F X ; Palenchar, Peter M. ; Runko, Suzan J. ; Twigg, Richard W. ; Dai, Guangwei ; Martienssen, Rob A. ; Benfey, Phillip N. ; Coruzzi, Gloria. / Expressed sequence tag analysis in Cycas, the most primitive living seed plant. In: Genome Biology. 2003 ; Vol. 4, No. 12.
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AU - Brenner, Eric D.

AU - Stevenson, Dennis W.

AU - McCombie, Richard W.

AU - Katari, Manpreet S.

AU - Rudd, Stephen A.

AU - Mayer, Klaus F X

AU - Palenchar, Peter M.

AU - Runko, Suzan J.

AU - Twigg, Richard W.

AU - Dai, Guangwei

AU - Martienssen, Rob A.

AU - Benfey, Phillip N.

AU - Coruzzi, Gloria

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