Exploring transporters within the small multidrug resistance family using solid-state NMR spectroscopy

Nathaniel Traaseth, James R. Banigan, Maureen Leninger

Research output: Contribution to journalArticle

Abstract

Small multidrug resistance (SMR) proteins comprise a family of bacterial secondary active transporters that confer drug resistance to antiseptics and antibiotics. EmrE has emerged as the model protein of the SMR family and an archetype to understand the ion-coupled transport mechanism. The importance of EmrE is further underscored by its proposed role as an evolutionary predecessor to larger transporters, which stems from the similarity of the antiparallel and asymmetric structure of the EmrE dimer with the inverted repeat fold of efflux pumps within other transport families such as the major facilitator superfamily. This review describes progress to reveal the atomic-scale structure and dynamics of SMR proteins determined under native-like conditions in lipid bilayers using solid-state NMR spectroscopy. The combination of MAS and oriented solid-state NMR approaches developed to study the SMR family serves as a framework for future efforts to uncover structural details of other secondary active transporters that are abundant in biology.

Original languageEnglish (US)
Pages (from-to)551-560
Number of pages10
JournaleMagRes
Volume4
Issue number2
DOIs
StatePublished - 2015

Fingerprint

P-Glycoproteins
Multiple Drug Resistance
Nuclear magnetic resonance spectroscopy
Magnetic Resonance Spectroscopy
Proteins
Lipid bilayers
Local Anti-Infective Agents
Dimers
Ion Transport
Nuclear magnetic resonance
Lipid Bilayers
Pumps
Antibiotics
Ions
Anti-Bacterial Agents
Drug Resistance
Pharmaceutical Preparations

Keywords

  • EmrE
  • Membrane proteins
  • Protein conformational dynamics
  • Small multidrug resistance family
  • Solid-state NMR spectroscopy

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy
  • Biomedical Engineering
  • Biochemistry
  • Radiology Nuclear Medicine and imaging

Cite this

Exploring transporters within the small multidrug resistance family using solid-state NMR spectroscopy. / Traaseth, Nathaniel; Banigan, James R.; Leninger, Maureen.

In: eMagRes, Vol. 4, No. 2, 2015, p. 551-560.

Research output: Contribution to journalArticle

Traaseth, Nathaniel ; Banigan, James R. ; Leninger, Maureen. / Exploring transporters within the small multidrug resistance family using solid-state NMR spectroscopy. In: eMagRes. 2015 ; Vol. 4, No. 2. pp. 551-560.
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