Exploring the molecular mechanism of stabilization of the adhesion domains of human CD2 by N-glycosylation

Xing Y. Wang, Chang G. Ji, John Zhang

Research output: Contribution to journalArticle

Abstract

N-Glycosylation is one of the most common cotranslational and post-translational modifications occurring in protein biosynthesis and plays a critical role in protein folding and structural diversification. Molecular dynamics studies of two benchmark systems, the NH2-terminal human CD2 adhesion domain (HsCD2ad), and the NH2-terminal rat CD2 adhesion domain (RnCD2ad) were carried out to investigate the energetic and dynamic effect of N-glycosylation on protein's stability. Our study revealed that N-glycosylation of HsCD2ad at the type I β-bulge turn strengthens the relevant hydrogen bonds, in particular, the hydrogen bond between Asn 65OD1-Thr67HG1. Dynamic cross correlation map analysis showed that nonglycosylated HsCD2ad has strong anticorrelated motions, whereas glycosylated HsCD2ad largely destroyed this anticorrelated motion. As a result, N-glycosylation energetically and dynamically stabilizes HsCD2ad. In contrast, N-glycosylation of RnCD2ad does not display observable effect on protein's stabilization. The current theoretical result is in excellent agreement with the recent thermodynamic experiment of Culyba et al. and indicates that enthalpy and entropy may both contribute to the stabilization of human CD2 by N-glycosylation.

Original languageEnglish (US)
Pages (from-to)11570-11577
Number of pages8
JournalJournal of Physical Chemistry B
Volume116
Issue number38
DOIs
StatePublished - Sep 27 2012

Fingerprint

Glycosylation
adhesion
Adhesion
Stabilization
stabilization
proteins
hydrogen bonds
Proteins
biosynthesis
Hydrogen bonds
folding
cross correlation
rats
Protein folding
enthalpy
Biosynthesis
entropy
molecular dynamics
thermodynamics
Molecular dynamics

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Materials Chemistry
  • Surfaces, Coatings and Films

Cite this

Exploring the molecular mechanism of stabilization of the adhesion domains of human CD2 by N-glycosylation. / Wang, Xing Y.; Ji, Chang G.; Zhang, John.

In: Journal of Physical Chemistry B, Vol. 116, No. 38, 27.09.2012, p. 11570-11577.

Research output: Contribution to journalArticle

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