Experimental antibacterial and mineralizing calcium phosphate-based treatment for dentin surfaces

Haijing Gu, Dindo Mijares, Ziming Zhao, Robert Boylan, Junqi Ling, Racquel Z. Legeros

Research output: Contribution to journalArticle

Abstract

Objectives: This study aimed to determine the efficacy of experimental calcium phosphate-based solutions (sCaP) containing fluoride (F), with and without zinc (Zn) ions on reducing susceptibility to acid dissolution and Streptococcus mutans (S. mutans) colonization of dentin surfaces.Methods: Dentin sections were treated with double distilled water (control) and with sCaP solutions differing in pH and in F- and/or Zn2+ ion concentrations. Solutions A (pH 7); B, C, and D (pH 5.5); solution C, twice Zn2+ and F- ion concentration compared to B; solution D is similar to C but without Zn2+. The dentin surfaces were characterized using scanning electron microscopy (SEM), x-ray diffraction, and Fourier Transform Infrared spectroscopy. Dissolution was determined in acidic buffer. Bacterial (S. mutans) attachment and growth were evaluated using SEM and Bioquant. Statistical analyses applied analysis of variance (ANOVA) and Duncan's multiple Range test.Results: Compared to control, dentin surfaces treated with sCaP solutions showed: (a) occluded dentin tubules; (b)reduced susceptibility to acid dissolution; and (c) Zn2+ ions were more effective than F- ions in inhibiting bacterial colonization.Significance: Acidic sCaP containing both F and Zn ions have mineralizing, acid resistance, and antibacterial effects and may be potentially useful as a strategy against dentin caries formation and progression.

Original languageEnglish (US)
Pages (from-to)783-790
Number of pages8
JournalJournal of Biomaterials Applications
Volume27
Issue number7
DOIs
StatePublished - Mar 2013

Fingerprint

Calcium phosphate
Ions
Dissolution
Zinc
Acid resistance
Scanning electron microscopy
Acids
Control surfaces
Analysis of variance (ANOVA)
Fluorides
Fourier transform infrared spectroscopy
calcium phosphate
Buffers
Diffraction
X rays
Water

Keywords

  • antibacteria
  • calcium phosphate
  • Dentin caries
  • mineralizing
  • S. mutans

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

Cite this

Experimental antibacterial and mineralizing calcium phosphate-based treatment for dentin surfaces. / Gu, Haijing; Mijares, Dindo; Zhao, Ziming; Boylan, Robert; Ling, Junqi; Legeros, Racquel Z.

In: Journal of Biomaterials Applications, Vol. 27, No. 7, 03.2013, p. 783-790.

Research output: Contribution to journalArticle

Gu, Haijing ; Mijares, Dindo ; Zhao, Ziming ; Boylan, Robert ; Ling, Junqi ; Legeros, Racquel Z. / Experimental antibacterial and mineralizing calcium phosphate-based treatment for dentin surfaces. In: Journal of Biomaterials Applications. 2013 ; Vol. 27, No. 7. pp. 783-790.
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