Evidence for redundancy but not trans factor-cis element coevolution in the regulation of Drosophila Yp genes

Fabio Piano, Michael J. Parisi, Roger Karess, Michael P. Kambysellis

Research output: Contribution to journalArticle

Abstract

In Drosophila melanogaster and the endemic Hawaiian species D. grimshawi three Yolk protein (Yp) genes are expressed in a similar sex- and tissue- specific pattern. In contrast, DNA sequence comparisons of promoter/enhancer regions show low levels of similarity. We tested the functional significance of these observations by transforming D. melanogaster with the genomic region that includes the divergently transcribed D. grimshawi DgYp1 and DgYp2 genes; we found that the introduced genes were expressed in female fat body and in ovaries but not in males. Moreover, we found D. grimshawi proteins in the hemolymph and accumulating in ovaries. Using reporter constructs we showed that the intergenic region from D. grimshawi was sufficient to drive accurate expression, but some low level of ectopic expression was seen in males. Transforming D. melanogaster with constructs bearing deletions within the D. grimshawi intergenic region revealed only subtle effects in the overall level of expression, suggesting a high level of redundancy. Testing mutants in the sex-specific regulator doublesex revealed that it is capable of repressing the DgYp genes in males. Together, these data show that D. melanogaster trans-acting factors can regulate the in vivo pattern of DgYp expression and support the notion of a redundant and complex system of cis-acting elements.

Original languageEnglish (US)
Pages (from-to)605-616
Number of pages12
JournalGenetics
Volume152
Issue number2
StatePublished - Jun 1999

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Drosophila Proteins
Egg Proteins
Drosophila melanogaster
Intergenic DNA
Genes
Ovary
Fat Body
Trans-Activators
Hemolymph
Genetic Promoter Regions
Proteins

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Evidence for redundancy but not trans factor-cis element coevolution in the regulation of Drosophila Yp genes. / Piano, Fabio; Parisi, Michael J.; Karess, Roger; Kambysellis, Michael P.

In: Genetics, Vol. 152, No. 2, 06.1999, p. 605-616.

Research output: Contribution to journalArticle

Piano, Fabio ; Parisi, Michael J. ; Karess, Roger ; Kambysellis, Michael P. / Evidence for redundancy but not trans factor-cis element coevolution in the regulation of Drosophila Yp genes. In: Genetics. 1999 ; Vol. 152, No. 2. pp. 605-616.
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