Evaluation of human polymorphonuclear behavior on textured titanium and calcium-phosphate coated surfaces

Camilla C G Moura, Juliana R. Machado, Marcos V. Silva, Denise B R Rodrigues, Darceny Zanetta-Barbosa, Ryo Jimbo, Nick Tovar, Paulo Coelho

Research output: Contribution to journalArticle

Abstract

Few studies have evaluated the effects of titanium (Ti) surface modifications on polymorphonuclear neutrophils (PMNs). Human PMNs' viability and release of key mediators - such as IL1β, IL6, TNFα, IL12, IL10, IL4, TGFβ1, IL8, IP-10, and Mig - were evaluated on three different Ti surface treatments: (1) machined Ti; (2) alumina-blasted and acid-etched Ti (AB/AE); and (3) calcium phosphate coating of 300-500 nm by ion beam onto the AB/AE Ti surface (CaP). A polystyrene surface was used as a negative control. The PMNs were purified from whole human blood and cultured for 6 h. Cell viability was determined by flow cytometry, and the supernatant was evaluated to determine the levels of cytokines and chemokines. Results showed that the percentage of viable cells was significantly lower on the CaP surface compared to the control (p < 0.05) relative to the other groups. No differences in the levels of IL8, MIG, and IP10 were detected between groups. Significantly higher levels of IL1β (p = 0.046) and TNFα (p = 0.016) were detected for the CaP surfaces compared to AB/AE surface only. The levels of IL4, IL10, and TGFβ1 secreted from the PMNs in the CaP group were significantly lower than in the control and machined groups (p < 0.05) that were statistically comparable to AB/AE. Overall, the addition of a thin CaP coating to the AB/AE Ti surface influenced the secretion profile of pro-inflammatory cytokines due to the higher release of pro-inflammatory cytokines (IL1β and TNFα) on these surfaces.

Original languageEnglish (US)
Article number035010
JournalBiomedical Materials (Bristol)
Volume8
Issue number3
DOIs
StatePublished - 2013

Fingerprint

Calcium phosphate
Titanium
Aluminum Oxide
Alumina
Neutrophils
Acids
Cytokines
Interleukin-8
Interleukin-4
Interleukin-10
Surface treatment
Phosphate coatings
Polystyrenes
Coatings
Interleukin-12
Flow cytometry
Chemokines
calcium phosphate
Interleukin-6
Cell Survival

ASJC Scopus subject areas

  • Chemistry (miscellaneous)
  • Biochemistry
  • Mechanics of Materials

Cite this

Evaluation of human polymorphonuclear behavior on textured titanium and calcium-phosphate coated surfaces. / Moura, Camilla C G; Machado, Juliana R.; Silva, Marcos V.; Rodrigues, Denise B R; Zanetta-Barbosa, Darceny; Jimbo, Ryo; Tovar, Nick; Coelho, Paulo.

In: Biomedical Materials (Bristol), Vol. 8, No. 3, 035010, 2013.

Research output: Contribution to journalArticle

Moura, Camilla C G ; Machado, Juliana R. ; Silva, Marcos V. ; Rodrigues, Denise B R ; Zanetta-Barbosa, Darceny ; Jimbo, Ryo ; Tovar, Nick ; Coelho, Paulo. / Evaluation of human polymorphonuclear behavior on textured titanium and calcium-phosphate coated surfaces. In: Biomedical Materials (Bristol). 2013 ; Vol. 8, No. 3.
@article{5f86c55eefc24b15a3b2ed7a2b8ffd87,
title = "Evaluation of human polymorphonuclear behavior on textured titanium and calcium-phosphate coated surfaces",
abstract = "Few studies have evaluated the effects of titanium (Ti) surface modifications on polymorphonuclear neutrophils (PMNs). Human PMNs' viability and release of key mediators - such as IL1β, IL6, TNFα, IL12, IL10, IL4, TGFβ1, IL8, IP-10, and Mig - were evaluated on three different Ti surface treatments: (1) machined Ti; (2) alumina-blasted and acid-etched Ti (AB/AE); and (3) calcium phosphate coating of 300-500 nm by ion beam onto the AB/AE Ti surface (CaP). A polystyrene surface was used as a negative control. The PMNs were purified from whole human blood and cultured for 6 h. Cell viability was determined by flow cytometry, and the supernatant was evaluated to determine the levels of cytokines and chemokines. Results showed that the percentage of viable cells was significantly lower on the CaP surface compared to the control (p < 0.05) relative to the other groups. No differences in the levels of IL8, MIG, and IP10 were detected between groups. Significantly higher levels of IL1β (p = 0.046) and TNFα (p = 0.016) were detected for the CaP surfaces compared to AB/AE surface only. The levels of IL4, IL10, and TGFβ1 secreted from the PMNs in the CaP group were significantly lower than in the control and machined groups (p < 0.05) that were statistically comparable to AB/AE. Overall, the addition of a thin CaP coating to the AB/AE Ti surface influenced the secretion profile of pro-inflammatory cytokines due to the higher release of pro-inflammatory cytokines (IL1β and TNFα) on these surfaces.",
author = "Moura, {Camilla C G} and Machado, {Juliana R.} and Silva, {Marcos V.} and Rodrigues, {Denise B R} and Darceny Zanetta-Barbosa and Ryo Jimbo and Nick Tovar and Paulo Coelho",
year = "2013",
doi = "10.1088/1748-6041/8/3/035010",
language = "English (US)",
volume = "8",
journal = "Biomedical Materials",
issn = "1748-6041",
publisher = "IOP Publishing Ltd.",
number = "3",

}

TY - JOUR

T1 - Evaluation of human polymorphonuclear behavior on textured titanium and calcium-phosphate coated surfaces

AU - Moura, Camilla C G

AU - Machado, Juliana R.

AU - Silva, Marcos V.

AU - Rodrigues, Denise B R

AU - Zanetta-Barbosa, Darceny

AU - Jimbo, Ryo

AU - Tovar, Nick

AU - Coelho, Paulo

PY - 2013

Y1 - 2013

N2 - Few studies have evaluated the effects of titanium (Ti) surface modifications on polymorphonuclear neutrophils (PMNs). Human PMNs' viability and release of key mediators - such as IL1β, IL6, TNFα, IL12, IL10, IL4, TGFβ1, IL8, IP-10, and Mig - were evaluated on three different Ti surface treatments: (1) machined Ti; (2) alumina-blasted and acid-etched Ti (AB/AE); and (3) calcium phosphate coating of 300-500 nm by ion beam onto the AB/AE Ti surface (CaP). A polystyrene surface was used as a negative control. The PMNs were purified from whole human blood and cultured for 6 h. Cell viability was determined by flow cytometry, and the supernatant was evaluated to determine the levels of cytokines and chemokines. Results showed that the percentage of viable cells was significantly lower on the CaP surface compared to the control (p < 0.05) relative to the other groups. No differences in the levels of IL8, MIG, and IP10 were detected between groups. Significantly higher levels of IL1β (p = 0.046) and TNFα (p = 0.016) were detected for the CaP surfaces compared to AB/AE surface only. The levels of IL4, IL10, and TGFβ1 secreted from the PMNs in the CaP group were significantly lower than in the control and machined groups (p < 0.05) that were statistically comparable to AB/AE. Overall, the addition of a thin CaP coating to the AB/AE Ti surface influenced the secretion profile of pro-inflammatory cytokines due to the higher release of pro-inflammatory cytokines (IL1β and TNFα) on these surfaces.

AB - Few studies have evaluated the effects of titanium (Ti) surface modifications on polymorphonuclear neutrophils (PMNs). Human PMNs' viability and release of key mediators - such as IL1β, IL6, TNFα, IL12, IL10, IL4, TGFβ1, IL8, IP-10, and Mig - were evaluated on three different Ti surface treatments: (1) machined Ti; (2) alumina-blasted and acid-etched Ti (AB/AE); and (3) calcium phosphate coating of 300-500 nm by ion beam onto the AB/AE Ti surface (CaP). A polystyrene surface was used as a negative control. The PMNs were purified from whole human blood and cultured for 6 h. Cell viability was determined by flow cytometry, and the supernatant was evaluated to determine the levels of cytokines and chemokines. Results showed that the percentage of viable cells was significantly lower on the CaP surface compared to the control (p < 0.05) relative to the other groups. No differences in the levels of IL8, MIG, and IP10 were detected between groups. Significantly higher levels of IL1β (p = 0.046) and TNFα (p = 0.016) were detected for the CaP surfaces compared to AB/AE surface only. The levels of IL4, IL10, and TGFβ1 secreted from the PMNs in the CaP group were significantly lower than in the control and machined groups (p < 0.05) that were statistically comparable to AB/AE. Overall, the addition of a thin CaP coating to the AB/AE Ti surface influenced the secretion profile of pro-inflammatory cytokines due to the higher release of pro-inflammatory cytokines (IL1β and TNFα) on these surfaces.

UR - http://www.scopus.com/inward/record.url?scp=84877781722&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84877781722&partnerID=8YFLogxK

U2 - 10.1088/1748-6041/8/3/035010

DO - 10.1088/1748-6041/8/3/035010

M3 - Article

VL - 8

JO - Biomedical Materials

JF - Biomedical Materials

SN - 1748-6041

IS - 3

M1 - 035010

ER -