Estimation of plasma very low density lipoprotein-cholesterol levels in uremia

E. N. Obineche, Abdishakur Abdulle, M. S. Lakhani, M. P.T. Gillett

Research output: Contribution to journalArticle

Abstract

Background. In investigations of plasma lipids and lipoproteins, very low density lipoprotein (VLDL) -cholesterol is often calculated from the level of plasma triacylglycerols (in mmol/1) divided by 2.2. This is then used to calculate low density lipoprotein (LDL)-cholesterol. This approach to quantifying lipoprotein levels is invalid for hypertriglyceridemic subjects and patients with type III or remnant hyperlipoproteinemia. It may also not give accurate results for subjects with other forms of dyslipoproteinemia, including patients with uremia or chronic renal failure. In the present study, a simple ultracentrifugation procedure was used to separate VLDL from normal and uremic plasma samples. The levels of VLDL-cholesterol were measured directly and compared with the calculated values. Methods. Fasted blood samples were collected from 31 control subjects and 99 uremic patients and analyzed for total, free, and high density lipoprotein (HDL)-cholesterol. An aliquot from each plasma was used to prepare VLDL by a single preparative ultracentrifuge spin, using a Beckman type 25 rotor. Total cholesterol was measured in the VLDL samples and compared with the values calculated from the plasma triacylglycerol levels. Results. Measurement of VLDL-cholesterol after preparative ultracentrifugation in the type 25 rotor was reproducible. There were significant correlations between the measured and the calculated VLDL-cholesterol levels for both groups of plasma samples. However, calculated values were consistently 20%-40% higher than the measured values in both groups. Conclusions. The simple method for direct measurement of VLDL-cholesterol described here is easy and reproducible, and avoids overestimation of this parameter in uremic plasma samples.

Original languageEnglish (US)
Pages (from-to)38-42
Number of pages5
JournalClinical and Experimental Nephrology
Volume6
Issue number1
DOIs
StatePublished - May 11 2002

Fingerprint

VLDL Cholesterol
Uremia
VLDL Lipoproteins
Ultracentrifugation
Lipoproteins
Triglycerides
Hyperlipoproteinemias
Dyslipidemias
LDL Cholesterol
HDL Cholesterol
Chronic Kidney Failure
Cholesterol
Lipids

Keywords

  • Lipoproteins
  • Ultracentrifugation
  • Uremia
  • VLDL-cholesterol

ASJC Scopus subject areas

  • Physiology
  • Nephrology
  • Physiology (medical)

Cite this

Estimation of plasma very low density lipoprotein-cholesterol levels in uremia. / Obineche, E. N.; Abdulle, Abdishakur; Lakhani, M. S.; Gillett, M. P.T.

In: Clinical and Experimental Nephrology, Vol. 6, No. 1, 11.05.2002, p. 38-42.

Research output: Contribution to journalArticle

Obineche, E. N. ; Abdulle, Abdishakur ; Lakhani, M. S. ; Gillett, M. P.T. / Estimation of plasma very low density lipoprotein-cholesterol levels in uremia. In: Clinical and Experimental Nephrology. 2002 ; Vol. 6, No. 1. pp. 38-42.
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abstract = "Background. In investigations of plasma lipids and lipoproteins, very low density lipoprotein (VLDL) -cholesterol is often calculated from the level of plasma triacylglycerols (in mmol/1) divided by 2.2. This is then used to calculate low density lipoprotein (LDL)-cholesterol. This approach to quantifying lipoprotein levels is invalid for hypertriglyceridemic subjects and patients with type III or remnant hyperlipoproteinemia. It may also not give accurate results for subjects with other forms of dyslipoproteinemia, including patients with uremia or chronic renal failure. In the present study, a simple ultracentrifugation procedure was used to separate VLDL from normal and uremic plasma samples. The levels of VLDL-cholesterol were measured directly and compared with the calculated values. Methods. Fasted blood samples were collected from 31 control subjects and 99 uremic patients and analyzed for total, free, and high density lipoprotein (HDL)-cholesterol. An aliquot from each plasma was used to prepare VLDL by a single preparative ultracentrifuge spin, using a Beckman type 25 rotor. Total cholesterol was measured in the VLDL samples and compared with the values calculated from the plasma triacylglycerol levels. Results. Measurement of VLDL-cholesterol after preparative ultracentrifugation in the type 25 rotor was reproducible. There were significant correlations between the measured and the calculated VLDL-cholesterol levels for both groups of plasma samples. However, calculated values were consistently 20{\%}-40{\%} higher than the measured values in both groups. Conclusions. The simple method for direct measurement of VLDL-cholesterol described here is easy and reproducible, and avoids overestimation of this parameter in uremic plasma samples.",
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AB - Background. In investigations of plasma lipids and lipoproteins, very low density lipoprotein (VLDL) -cholesterol is often calculated from the level of plasma triacylglycerols (in mmol/1) divided by 2.2. This is then used to calculate low density lipoprotein (LDL)-cholesterol. This approach to quantifying lipoprotein levels is invalid for hypertriglyceridemic subjects and patients with type III or remnant hyperlipoproteinemia. It may also not give accurate results for subjects with other forms of dyslipoproteinemia, including patients with uremia or chronic renal failure. In the present study, a simple ultracentrifugation procedure was used to separate VLDL from normal and uremic plasma samples. The levels of VLDL-cholesterol were measured directly and compared with the calculated values. Methods. Fasted blood samples were collected from 31 control subjects and 99 uremic patients and analyzed for total, free, and high density lipoprotein (HDL)-cholesterol. An aliquot from each plasma was used to prepare VLDL by a single preparative ultracentrifuge spin, using a Beckman type 25 rotor. Total cholesterol was measured in the VLDL samples and compared with the values calculated from the plasma triacylglycerol levels. Results. Measurement of VLDL-cholesterol after preparative ultracentrifugation in the type 25 rotor was reproducible. There were significant correlations between the measured and the calculated VLDL-cholesterol levels for both groups of plasma samples. However, calculated values were consistently 20%-40% higher than the measured values in both groups. Conclusions. The simple method for direct measurement of VLDL-cholesterol described here is easy and reproducible, and avoids overestimation of this parameter in uremic plasma samples.

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