Establishment of a critical-sized alveolar defect in the rat

A model for human gingivoperiosteoplasty

Phuong D. Nguyen, Clarence D. Lin, Alexander C. Allori, John Ricci, Pierre B. Saadeh, Stephen M. Warren

Research output: Contribution to journalArticle

Abstract

BACKGROUND:: Despite technical advancement, treatment of congenital alveolar clefts has remained controversial. Currently, primary alveolar cleft repair (i.e., gingivoperiosteoplasty) has a 41 to 73 percent success rate. However, the remaining patients have persistent alveolar bone defects requiring secondary grafting procedures. Morbidity of secondary procedures includes pain, graft resorption, extrusion or infection, and graft or tooth loss. The authors present a novel rat alveolar defect model designed to facilitate investigation of therapeutics aimed at improving bone formation following primary alveolar cleft repair in humans. METHODS:: Sixteen 8-week-old Sprague-Dawley rats underwent creation of a 7 × 4 × 3-mm complete alveolar defect from the maxillary incisors to the zygomatic arch. Four animals were humanely killed at each of the following time points: 0, 4, 8, and 12 weeks. Morphometric analysis of the alveolar defect was determined by means of micro-computed tomography and histology. RESULTS:: Micro-computed tomography demonstrated that new bone filled 43 ± 5.6 percent of the alveolar defect at 4 weeks, 53 ± 8.3 percent at 8 weeks, and 48 ± 3.5 percent at 12 weeks. Histologically, at 4 weeks, proliferating fibroblasts and polymorphonuclear cells were scattered throughout the disorganized collagen in the intercalary gap. By 8 weeks, nascent woven bone spicules extended from the edges of the defect. At 12 weeks, the woven spicules had remodeled, with scant additional bone deposition. CONCLUSION:: This model creates a critical-size alveolar defect that is similar in size and location to human alveolar defects and is suitable for studying proposed therapeutics.

Original languageEnglish (US)
Pages (from-to)817-825
Number of pages9
JournalPlastic and Reconstructive Surgery
Volume123
Issue number3
DOIs
StatePublished - Mar 2009

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Bone and Bones
Tomography
Zygoma
Transplants
Tooth Loss
Incisor
Osteogenesis
Sprague Dawley Rats
Histology
Collagen
Therapeutics
Fibroblasts
Morbidity
Pain
Infection

ASJC Scopus subject areas

  • Surgery

Cite this

Establishment of a critical-sized alveolar defect in the rat : A model for human gingivoperiosteoplasty. / Nguyen, Phuong D.; Lin, Clarence D.; Allori, Alexander C.; Ricci, John; Saadeh, Pierre B.; Warren, Stephen M.

In: Plastic and Reconstructive Surgery, Vol. 123, No. 3, 03.2009, p. 817-825.

Research output: Contribution to journalArticle

Nguyen, Phuong D. ; Lin, Clarence D. ; Allori, Alexander C. ; Ricci, John ; Saadeh, Pierre B. ; Warren, Stephen M. / Establishment of a critical-sized alveolar defect in the rat : A model for human gingivoperiosteoplasty. In: Plastic and Reconstructive Surgery. 2009 ; Vol. 123, No. 3. pp. 817-825.
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