ErbB2 Activation of ESX gene expression

Richard M. Neve, Bauke Ylstra, Chuan Hsiung Chang, Donna Albertson, Christopher C. Benz

Research output: Contribution to journalArticle

Abstract

Various members of the Ets multigene family exhibit diverse roles in development, cell differentiation, tissue-specific gene expression and human malignancy. In the search for Ets factors involved in mammary gland development and malignancy, ESX was found to be upregulated in a subset of breast tumours and cell lines. We report the transcriptional regulation of ESX in epithelial breast cancer cells. Transient reporter assays using the ESX promoter show that ESX transcription is regulated by ErbB receptor signalling. In cell lines and in 45 primary ductal breast cancers we show that ESX transcript expression significantly correlates with ErbB2 transcript levels. Moreover, expression of ErbB2 in cells upregulates ESX promoter activity while inhibition of ErbB2 or its downstream signaling pathways decrease both ESX promoter activity and endogenous ESX protein levels. These results indicate that the ESX promoter represents a transcriptional target of ErbB2, and ESX expression may represent a downstream mediator of ErbB2 signaling and ErbB2-induced gene expression.

Original languageEnglish (US)
Pages (from-to)3934-3938
Number of pages5
JournalOncogene
Volume21
Issue number24
DOIs
StatePublished - May 30 2002

Fingerprint

Breast Neoplasms
Gene Expression
erbB-2 Genes
Human Mammary Glands
Multigene Family
Tumor Cell Line
Cell Differentiation
Neoplasms
Up-Regulation
Cell Line
Proteins
ErbB Receptors

Keywords

  • Breast cancer
  • ErbB2
  • ESX
  • Ets

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

Neve, R. M., Ylstra, B., Chang, C. H., Albertson, D., & Benz, C. C. (2002). ErbB2 Activation of ESX gene expression. Oncogene, 21(24), 3934-3938. https://doi.org/10.1038/sj/onc/1205503

ErbB2 Activation of ESX gene expression. / Neve, Richard M.; Ylstra, Bauke; Chang, Chuan Hsiung; Albertson, Donna; Benz, Christopher C.

In: Oncogene, Vol. 21, No. 24, 30.05.2002, p. 3934-3938.

Research output: Contribution to journalArticle

Neve, RM, Ylstra, B, Chang, CH, Albertson, D & Benz, CC 2002, 'ErbB2 Activation of ESX gene expression', Oncogene, vol. 21, no. 24, pp. 3934-3938. https://doi.org/10.1038/sj/onc/1205503
Neve RM, Ylstra B, Chang CH, Albertson D, Benz CC. ErbB2 Activation of ESX gene expression. Oncogene. 2002 May 30;21(24):3934-3938. https://doi.org/10.1038/sj/onc/1205503
Neve, Richard M. ; Ylstra, Bauke ; Chang, Chuan Hsiung ; Albertson, Donna ; Benz, Christopher C. / ErbB2 Activation of ESX gene expression. In: Oncogene. 2002 ; Vol. 21, No. 24. pp. 3934-3938.
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