Equilibrium unfolding thermodynamics of β2-microglobulin analyzed through native-state H/D exchange

Enrico Rennella, Alessandra Corazza, Federico Fogolari, Paolo Viglino, Sofia Giorgetti, Monica Stoppini, Vittorio Bellotti, Gennaro Esposito

Research output: Contribution to journalArticle

Abstract

The exchange rates for the amide hydrogens of β2- microglobulin, the protein responsible for dialysis-related amyloidosis, were measured under native conditions at different temperatures ranging from 301 to 315 K. The pattern of protection factors within different regions of the protein correlates well with the hydrogen-bonding pattern of the deposited structures. Analysis of the exchange rates indicates the presence of mixed EX1- and EX2-limit mechanisms. The measured parameters are consistent with a two-process model in which two competing pathways, i.e., global unfolding in the core region and partial openings of the native state, determine the observed exchange rates. These findings are analyzed with respect to the amyloidogenic properties of the protein.

Original languageEnglish (US)
Pages (from-to)169-179
Number of pages11
JournalBiophysical Journal
Volume96
Issue number1
DOIs
StatePublished - Jan 7 2009

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Thermodynamics
Amyloidogenic Proteins
Amyloidosis
Hydrogen Bonding
Amides
Dialysis
Hydrogen
Proteins
Temperature

ASJC Scopus subject areas

  • Biophysics

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Equilibrium unfolding thermodynamics of β2-microglobulin analyzed through native-state H/D exchange. / Rennella, Enrico; Corazza, Alessandra; Fogolari, Federico; Viglino, Paolo; Giorgetti, Sofia; Stoppini, Monica; Bellotti, Vittorio; Esposito, Gennaro.

In: Biophysical Journal, Vol. 96, No. 1, 07.01.2009, p. 169-179.

Research output: Contribution to journalArticle

Rennella, E, Corazza, A, Fogolari, F, Viglino, P, Giorgetti, S, Stoppini, M, Bellotti, V & Esposito, G 2009, 'Equilibrium unfolding thermodynamics of β2-microglobulin analyzed through native-state H/D exchange', Biophysical Journal, vol. 96, no. 1, pp. 169-179. https://doi.org/10.1529/biophysj.108.142448
Rennella, Enrico ; Corazza, Alessandra ; Fogolari, Federico ; Viglino, Paolo ; Giorgetti, Sofia ; Stoppini, Monica ; Bellotti, Vittorio ; Esposito, Gennaro. / Equilibrium unfolding thermodynamics of β2-microglobulin analyzed through native-state H/D exchange. In: Biophysical Journal. 2009 ; Vol. 96, No. 1. pp. 169-179.
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