Epigenetics, development, and cancer: Zebrafish make their ARK

Raksha Mudbhary, Kirsten Sadler Edepli

Research output: Contribution to journalReview article

Abstract

Zebrafish embryos are an exceptional system for studying vertebrate development. Historically, studies using zebrafish to uncover key players in developmentally regulated gene expression have entailed detailed analysis of transcription factors. It is now apparent that epigenetic modifications of both DNA and histone tails are equally important in the regulation of gene expression during development. As such, blocking the function of key epigenetic modifiers impairs development, albeit with surprising tissue specificity. For instance, DNA methylation is an important epigenetic mark that is depleted in embryos lacking dnmt1 and uhrf1. These embryos display developmental defects in the eye, liver, pancreas, and larval lethality. Interestingly, human tumors derived from these same organs have aberrant changes in DNA methylation and altered expression of genes that are thought to contribute to formation of these cancers. These observations have provided a mechanistic basis for treating cancer with drugs that block the enzymes that facilitate DNA and histone modifications. Thus, it is important to understand the consequences of targeting these factors in a whole animal. We review the use of zebrafish for probing the genetic, cellular, and physiological response to alterations in the epigenome and highlight exciting data illustrating that epigenetic studies using zebrafish can inform and impact cancer biology.

Original languageEnglish (US)
Pages (from-to)194-203
Number of pages10
JournalBirth Defects Research Part C - Embryo Today: Reviews
Volume93
Issue number2
DOIs
StatePublished - Jun 1 2011

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Zebrafish
Epigenomics
Embryonic Structures
DNA Methylation
Neoplasms
Histone Code
Gene Expression
Organ Specificity
DNA
Gene Expression Regulation
Histones
Vertebrates
Tail
Pancreas
Transcription Factors
Liver
Enzymes
Pharmaceutical Preparations

Keywords

  • Development
  • DNA methylation
  • DNMT1
  • Histone acetylation
  • Histone methylation
  • UHRF1

ASJC Scopus subject areas

  • Embryology
  • Developmental Biology

Cite this

Epigenetics, development, and cancer : Zebrafish make their ARK. / Mudbhary, Raksha; Sadler Edepli, Kirsten.

In: Birth Defects Research Part C - Embryo Today: Reviews, Vol. 93, No. 2, 01.06.2011, p. 194-203.

Research output: Contribution to journalReview article

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