Epidermal growth factor downregulates the expression of SH-PTP2.

Angela Kamer, S. A. Hoghooghi, C. Liebow

Research output: Contribution to journalArticle

Abstract

Protein phosphorylation/dephosphorylation on tyrosine residues are regulated by tyrosine kinases/phosphatases. The tyrosine phosphatase SH-PTP2 (PTP1D, PTP2C) interacts physically through its SH2 domain with phosphorylated epidermal growth factor receptor (EGFR). In KB cells, an oral epidermoid carcinoma, high epidermal growth factor (EGF) concentrations (10-9, 10-8 and 10-7 M) downregulate its receptor for the duration of the incubation with EGF. Thus, it was hypothesized that in KB cells, SH-PTP2 expression would also be downregulated by high EGF concentrations. This hypothesis was investigated by incubating the KB cells with increasing concentrations of EGF (0, 10-11, 10-10, 10-9, 10-8, 10-7 M) and by evaluating the expression of SH-PTP2 protein under these conditions. This study showed that EGF 10-7 and 10-8 M significantly decreased SH-PTP2 protein expression compared to controls. EGF 10-10 and 10-11 M did not change the expression of SH-PTP2 protein. We conclude that high EGF concentrations downregulate the expression of SH-PTP2 protein.

Original languageEnglish (US)
Pages (from-to)735-739
Number of pages5
JournalInternational Journal of Molecular Medicine
Volume1
Issue number4
StatePublished - Apr 1998

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Epidermal Growth Factor
Down-Regulation
KB Cells
Proteins
Phosphoric Monoester Hydrolases
Tyrosine
src Homology Domains
Epidermal Growth Factor Receptor
Protein-Tyrosine Kinases
Squamous Cell Carcinoma
Phosphorylation

ASJC Scopus subject areas

  • Genetics

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Epidermal growth factor downregulates the expression of SH-PTP2. / Kamer, Angela; Hoghooghi, S. A.; Liebow, C.

In: International Journal of Molecular Medicine, Vol. 1, No. 4, 04.1998, p. 735-739.

Research output: Contribution to journalArticle

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abstract = "Protein phosphorylation/dephosphorylation on tyrosine residues are regulated by tyrosine kinases/phosphatases. The tyrosine phosphatase SH-PTP2 (PTP1D, PTP2C) interacts physically through its SH2 domain with phosphorylated epidermal growth factor receptor (EGFR). In KB cells, an oral epidermoid carcinoma, high epidermal growth factor (EGF) concentrations (10-9, 10-8 and 10-7 M) downregulate its receptor for the duration of the incubation with EGF. Thus, it was hypothesized that in KB cells, SH-PTP2 expression would also be downregulated by high EGF concentrations. This hypothesis was investigated by incubating the KB cells with increasing concentrations of EGF (0, 10-11, 10-10, 10-9, 10-8, 10-7 M) and by evaluating the expression of SH-PTP2 protein under these conditions. This study showed that EGF 10-7 and 10-8 M significantly decreased SH-PTP2 protein expression compared to controls. EGF 10-10 and 10-11 M did not change the expression of SH-PTP2 protein. We conclude that high EGF concentrations downregulate the expression of SH-PTP2 protein.",
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