Enhancing protease activity assay in droplet-based microfluidics using a biomolecule concentrator

Chia Hung Chen, Aniruddh Sarkar, Rafael Song, Miles A. Miller, Sung Jae Kim, Linda G. Griffith, Douglas A. Lauffenburger, Jongyoon Han

    Research output: Contribution to journalArticle

    Abstract

    We introduce an integrated microfluidic device consisting of a biomolecule concentrator and a microdroplet generator, which enhances the limited sensitivity of low-abundance enzyme assays by concentrating biomolecules before encapsulating them into droplet microreactors. We used this platform to detect ultralow levels of matrix metalloproteinases (MMPs) from diluted cellular supernatant and showed that it significantly (∼10-fold) reduced the time required to complete the assay and the sample volume used.

    Original languageEnglish (US)
    Pages (from-to)10368-10371
    Number of pages4
    JournalJournal of the American Chemical Society
    Volume133
    Issue number27
    DOIs
    StatePublished - Jul 13 2011

    Fingerprint

    Lab-On-A-Chip Devices
    Microfluidics
    Enzyme Assays
    Biomolecules
    Matrix Metalloproteinases
    Assays
    Peptide Hydrolases
    Enzymes

    ASJC Scopus subject areas

    • Catalysis
    • Chemistry(all)
    • Biochemistry
    • Colloid and Surface Chemistry

    Cite this

    Chen, C. H., Sarkar, A., Song, R., Miller, M. A., Kim, S. J., Griffith, L. G., ... Han, J. (2011). Enhancing protease activity assay in droplet-based microfluidics using a biomolecule concentrator. Journal of the American Chemical Society, 133(27), 10368-10371. https://doi.org/10.1021/ja2036628

    Enhancing protease activity assay in droplet-based microfluidics using a biomolecule concentrator. / Chen, Chia Hung; Sarkar, Aniruddh; Song, Rafael; Miller, Miles A.; Kim, Sung Jae; Griffith, Linda G.; Lauffenburger, Douglas A.; Han, Jongyoon.

    In: Journal of the American Chemical Society, Vol. 133, No. 27, 13.07.2011, p. 10368-10371.

    Research output: Contribution to journalArticle

    Chen, CH, Sarkar, A, Song, R, Miller, MA, Kim, SJ, Griffith, LG, Lauffenburger, DA & Han, J 2011, 'Enhancing protease activity assay in droplet-based microfluidics using a biomolecule concentrator', Journal of the American Chemical Society, vol. 133, no. 27, pp. 10368-10371. https://doi.org/10.1021/ja2036628
    Chen, Chia Hung ; Sarkar, Aniruddh ; Song, Rafael ; Miller, Miles A. ; Kim, Sung Jae ; Griffith, Linda G. ; Lauffenburger, Douglas A. ; Han, Jongyoon. / Enhancing protease activity assay in droplet-based microfluidics using a biomolecule concentrator. In: Journal of the American Chemical Society. 2011 ; Vol. 133, No. 27. pp. 10368-10371.
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    AU - Lauffenburger, Douglas A.

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