Enhancing protease activity assay in droplet-based microfluidics using a biomolecule concentrator

Chia Hung Chen, Aniruddh Sarkar, Rafael Song, Miles A. Miller, Sung Jae Kim, Linda G. Griffith, Douglas A. Lauffenburger, Jongyoon Han

Research output: Contribution to journalArticle

Abstract

We introduce an integrated microfluidic device consisting of a biomolecule concentrator and a microdroplet generator, which enhances the limited sensitivity of low-abundance enzyme assays by concentrating biomolecules before encapsulating them into droplet microreactors. We used this platform to detect ultralow levels of matrix metalloproteinases (MMPs) from diluted cellular supernatant and showed that it significantly (∼10-fold) reduced the time required to complete the assay and the sample volume used.

Original languageEnglish (US)
Pages (from-to)10368-10371
Number of pages4
JournalJournal of the American Chemical Society
Volume133
Issue number27
DOIs
StatePublished - Jul 13 2011

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Lab-On-A-Chip Devices
Microfluidics
Enzyme Assays
Biomolecules
Matrix Metalloproteinases
Assays
Peptide Hydrolases
Enzymes

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Enhancing protease activity assay in droplet-based microfluidics using a biomolecule concentrator. / Chen, Chia Hung; Sarkar, Aniruddh; Song, Rafael; Miller, Miles A.; Kim, Sung Jae; Griffith, Linda G.; Lauffenburger, Douglas A.; Han, Jongyoon.

In: Journal of the American Chemical Society, Vol. 133, No. 27, 13.07.2011, p. 10368-10371.

Research output: Contribution to journalArticle

Chen, CH, Sarkar, A, Song, R, Miller, MA, Kim, SJ, Griffith, LG, Lauffenburger, DA & Han, J 2011, 'Enhancing protease activity assay in droplet-based microfluidics using a biomolecule concentrator', Journal of the American Chemical Society, vol. 133, no. 27, pp. 10368-10371. https://doi.org/10.1021/ja2036628
Chen, Chia Hung ; Sarkar, Aniruddh ; Song, Rafael ; Miller, Miles A. ; Kim, Sung Jae ; Griffith, Linda G. ; Lauffenburger, Douglas A. ; Han, Jongyoon. / Enhancing protease activity assay in droplet-based microfluidics using a biomolecule concentrator. In: Journal of the American Chemical Society. 2011 ; Vol. 133, No. 27. pp. 10368-10371.
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