Enhanced synaptophysin immunoreactivity in rat hippocampal culture by 5-HT1A agonist, S100b, and corticosteroid receptor agonists

Mayumi Nishi, Patricia M. Whitaker-Azmitia, Efrain C. Azmitia

Research output: Contribution to journalArticle

Abstract

Serotonin (5-HT) has been shown to modulate brain maturation during development and adult plasticity. This effect in the whole animal may be due to activation of 5-HT1A receptors and a corresponding increases in S100b and corticosterone. Synaptophysin, an integral protein of the synaptic vesicle membrane that correlates with synaptic density and neurotransmitter release, is reduced by depletion of 5-HT in the cortex and hippocampus of the adult rat. Injections of a 5-HT1A agonist or dexamethasone can reverse the loss of synaptophysin immunoreactivity (IR). In this study we used morphometric analysis of synaptophysin-IR to study the effects of the 5-HT1A agonist, ipsapirone, and the neuronal extension factor, S100b on hippocampal neurons grown in a serum and steroid free media. Both compounds increased the synaptophysin-IR at doses previously established to be highly specific. Ipsapirone (10-9 M) was more effective on neuronal cell bodies staining and S100b (10 ng/ml) was more effective in increasing the number of synaptophysin-IR varicosities on neuronal processes. In addition both types of corticosteroid receptor agonists, at previously established specific doses, Ru28362 (10-8 M) and aldosterone (10-9 M) produced smaller increases compared to control groups in both the cell body staining and the number of varicosities. The effect of these differentiating factors on the expression of synaptophysin-IR suggests multiple regulation sites for producing and maintaining pre-synaptic elements in the brain.

Original languageEnglish (US)
Pages (from-to)1-9
Number of pages9
JournalSynapse
Volume23
Issue number1
StatePublished - May 1996

Fingerprint

Serotonin 5-HT1 Receptor Agonists
Synaptophysin
Steroid Receptors
Serotonin
Staining and Labeling
Synaptic Membranes
Receptor, Serotonin, 5-HT1A
Synaptic Vesicles
Serum-Free Culture Media
Brain
Corticosterone
Aldosterone
Dexamethasone
Neurotransmitter Agents
Hippocampus
Steroids
Neurons
Control Groups
Injections

Keywords

  • Aldosterone
  • Differentiation
  • Ipsapirone
  • Ru28362
  • Synaptogenesis
  • Varicosity

ASJC Scopus subject areas

  • Physiology
  • Neuroscience(all)
  • Pharmacology

Cite this

Enhanced synaptophysin immunoreactivity in rat hippocampal culture by 5-HT1A agonist, S100b, and corticosteroid receptor agonists. / Nishi, Mayumi; Whitaker-Azmitia, Patricia M.; Azmitia, Efrain C.

In: Synapse, Vol. 23, No. 1, 05.1996, p. 1-9.

Research output: Contribution to journalArticle

@article{56980286480a4a3a82f37f50bc940462,
title = "Enhanced synaptophysin immunoreactivity in rat hippocampal culture by 5-HT1A agonist, S100b, and corticosteroid receptor agonists",
abstract = "Serotonin (5-HT) has been shown to modulate brain maturation during development and adult plasticity. This effect in the whole animal may be due to activation of 5-HT1A receptors and a corresponding increases in S100b and corticosterone. Synaptophysin, an integral protein of the synaptic vesicle membrane that correlates with synaptic density and neurotransmitter release, is reduced by depletion of 5-HT in the cortex and hippocampus of the adult rat. Injections of a 5-HT1A agonist or dexamethasone can reverse the loss of synaptophysin immunoreactivity (IR). In this study we used morphometric analysis of synaptophysin-IR to study the effects of the 5-HT1A agonist, ipsapirone, and the neuronal extension factor, S100b on hippocampal neurons grown in a serum and steroid free media. Both compounds increased the synaptophysin-IR at doses previously established to be highly specific. Ipsapirone (10-9 M) was more effective on neuronal cell bodies staining and S100b (10 ng/ml) was more effective in increasing the number of synaptophysin-IR varicosities on neuronal processes. In addition both types of corticosteroid receptor agonists, at previously established specific doses, Ru28362 (10-8 M) and aldosterone (10-9 M) produced smaller increases compared to control groups in both the cell body staining and the number of varicosities. The effect of these differentiating factors on the expression of synaptophysin-IR suggests multiple regulation sites for producing and maintaining pre-synaptic elements in the brain.",
keywords = "Aldosterone, Differentiation, Ipsapirone, Ru28362, Synaptogenesis, Varicosity",
author = "Mayumi Nishi and Whitaker-Azmitia, {Patricia M.} and Azmitia, {Efrain C.}",
year = "1996",
month = "5",
language = "English (US)",
volume = "23",
pages = "1--9",
journal = "Synapse",
issn = "0887-4476",
publisher = "Wiley-Liss Inc.",
number = "1",

}

TY - JOUR

T1 - Enhanced synaptophysin immunoreactivity in rat hippocampal culture by 5-HT1A agonist, S100b, and corticosteroid receptor agonists

AU - Nishi, Mayumi

AU - Whitaker-Azmitia, Patricia M.

AU - Azmitia, Efrain C.

PY - 1996/5

Y1 - 1996/5

N2 - Serotonin (5-HT) has been shown to modulate brain maturation during development and adult plasticity. This effect in the whole animal may be due to activation of 5-HT1A receptors and a corresponding increases in S100b and corticosterone. Synaptophysin, an integral protein of the synaptic vesicle membrane that correlates with synaptic density and neurotransmitter release, is reduced by depletion of 5-HT in the cortex and hippocampus of the adult rat. Injections of a 5-HT1A agonist or dexamethasone can reverse the loss of synaptophysin immunoreactivity (IR). In this study we used morphometric analysis of synaptophysin-IR to study the effects of the 5-HT1A agonist, ipsapirone, and the neuronal extension factor, S100b on hippocampal neurons grown in a serum and steroid free media. Both compounds increased the synaptophysin-IR at doses previously established to be highly specific. Ipsapirone (10-9 M) was more effective on neuronal cell bodies staining and S100b (10 ng/ml) was more effective in increasing the number of synaptophysin-IR varicosities on neuronal processes. In addition both types of corticosteroid receptor agonists, at previously established specific doses, Ru28362 (10-8 M) and aldosterone (10-9 M) produced smaller increases compared to control groups in both the cell body staining and the number of varicosities. The effect of these differentiating factors on the expression of synaptophysin-IR suggests multiple regulation sites for producing and maintaining pre-synaptic elements in the brain.

AB - Serotonin (5-HT) has been shown to modulate brain maturation during development and adult plasticity. This effect in the whole animal may be due to activation of 5-HT1A receptors and a corresponding increases in S100b and corticosterone. Synaptophysin, an integral protein of the synaptic vesicle membrane that correlates with synaptic density and neurotransmitter release, is reduced by depletion of 5-HT in the cortex and hippocampus of the adult rat. Injections of a 5-HT1A agonist or dexamethasone can reverse the loss of synaptophysin immunoreactivity (IR). In this study we used morphometric analysis of synaptophysin-IR to study the effects of the 5-HT1A agonist, ipsapirone, and the neuronal extension factor, S100b on hippocampal neurons grown in a serum and steroid free media. Both compounds increased the synaptophysin-IR at doses previously established to be highly specific. Ipsapirone (10-9 M) was more effective on neuronal cell bodies staining and S100b (10 ng/ml) was more effective in increasing the number of synaptophysin-IR varicosities on neuronal processes. In addition both types of corticosteroid receptor agonists, at previously established specific doses, Ru28362 (10-8 M) and aldosterone (10-9 M) produced smaller increases compared to control groups in both the cell body staining and the number of varicosities. The effect of these differentiating factors on the expression of synaptophysin-IR suggests multiple regulation sites for producing and maintaining pre-synaptic elements in the brain.

KW - Aldosterone

KW - Differentiation

KW - Ipsapirone

KW - Ru28362

KW - Synaptogenesis

KW - Varicosity

UR - http://www.scopus.com/inward/record.url?scp=0029876165&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029876165&partnerID=8YFLogxK

M3 - Article

VL - 23

SP - 1

EP - 9

JO - Synapse

JF - Synapse

SN - 0887-4476

IS - 1

ER -