Engineering light-gated ion channels

Matthew R. Banghart, Matthew Volgraf, Dirk Trauner

Research output: Contribution to journalReview article

Abstract

Ion channels are gated by a variety of stimuli, including ligands, voltage, membrane tension, temperature, and even light. Natural gates can be altered and augmented using synthetic chemistry and molecular biology to develop channels with completely new functional properties. Light-sensitive channels are particularly attractive because optical manipulation offers a high degree of spatial and temporal control. Over the last few decades, several channels have been successfully rendered responsive to light, including the nicotinic acetylcholine receptor, gramicidin A, a voltage-gated potassium channel, an ionotropic glutamate receptor, α-hemolysin, and a mechanosensitive channel. Very recently, naturally occurring light-gated cation channels have been discovered. This review covers the molecular principles that guide the engineering of light-gated ion channels for applications in biology and medicine.

Original languageEnglish (US)
Pages (from-to)15129-15141
Number of pages13
JournalBiochemistry
Volume45
Issue number51
DOIs
StatePublished - Dec 26 2006

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Ion Channels
Light
Synthetic Biology
Ionotropic Glutamate Receptors
Gramicidin
Voltage-Gated Potassium Channels
Hemolysin Proteins
Molecular biology
Nicotinic Receptors
Medicine
Cations
Molecular Biology
Ligands
Membranes
Temperature
Electric potential

ASJC Scopus subject areas

  • Biochemistry

Cite this

Banghart, M. R., Volgraf, M., & Trauner, D. (2006). Engineering light-gated ion channels. Biochemistry, 45(51), 15129-15141. https://doi.org/10.1021/bi0618058

Engineering light-gated ion channels. / Banghart, Matthew R.; Volgraf, Matthew; Trauner, Dirk.

In: Biochemistry, Vol. 45, No. 51, 26.12.2006, p. 15129-15141.

Research output: Contribution to journalReview article

Banghart, MR, Volgraf, M & Trauner, D 2006, 'Engineering light-gated ion channels', Biochemistry, vol. 45, no. 51, pp. 15129-15141. https://doi.org/10.1021/bi0618058
Banghart MR, Volgraf M, Trauner D. Engineering light-gated ion channels. Biochemistry. 2006 Dec 26;45(51):15129-15141. https://doi.org/10.1021/bi0618058
Banghart, Matthew R. ; Volgraf, Matthew ; Trauner, Dirk. / Engineering light-gated ion channels. In: Biochemistry. 2006 ; Vol. 45, No. 51. pp. 15129-15141.
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