Engineering endochondral bone

In vitro studies

Serafim M. Oliveira, Isabel F. Amaral, Mário A. Barbosa, Cristina Teixeira

Research output: Contribution to journalArticle

Abstract

Chitosan scaffolds have been shown to possess biological and mechanical properties suitable for tissue engineering and clinical applications. In the present work, chitosan sponges were evaluated regarding their ability to support cartilage cell proliferation and maturation, which are the first steps in endochondral bone formation. Chitosan sponges were seeded with chondrocytes isolated from chicken embryo sterna. Chondrocyte/chitosan constructs were cultured for 20 days, and treated with retinoic acid (RA) to induce chondrocyte maturation and matrix synthesis. At different time points, samples were collected for microscopic, histological, biochemical, and mechanical analyses. Results show chondrocyte attachment, proliferation, and abundant matrix synthesis, completely obliterating the pores of the sponges. RA treatment caused chondrocyte hypertrophy, characterized by the presence of type X collagen in the extracellular matrix and increased alkaline phosphatase activity. In addition, hypertrophy markedly changed the mechanical properties of the chondrocyte/chitosan constructs. In conclusion, we have developed chitosan sponges with adequate pore structure and mechanical properties to serve as a support for hypertrophic chondrocytes. In parallel studies, we have evaluated the ability of this mature cartilage scaffold to induce endochondral ossification.

Original languageEnglish (US)
Pages (from-to)625-634
Number of pages10
JournalTissue Engineering - Part A
Volume15
Issue number3
DOIs
StatePublished - Mar 1 2009

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Chitosan
Chondrocytes
Bone
Bone and Bones
Porifera
Cartilage
Tretinoin
Scaffolds
Mechanical properties
Osteogenesis
Hypertrophy
Collagen Type X
Acids
Phosphatases
Cell proliferation
Sternum
Pore structure
Tissue engineering
Collagen
Tissue Engineering

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Biomedical Engineering
  • Biomaterials

Cite this

Engineering endochondral bone : In vitro studies. / Oliveira, Serafim M.; Amaral, Isabel F.; Barbosa, Mário A.; Teixeira, Cristina.

In: Tissue Engineering - Part A, Vol. 15, No. 3, 01.03.2009, p. 625-634.

Research output: Contribution to journalArticle

Oliveira, Serafim M. ; Amaral, Isabel F. ; Barbosa, Mário A. ; Teixeira, Cristina. / Engineering endochondral bone : In vitro studies. In: Tissue Engineering - Part A. 2009 ; Vol. 15, No. 3. pp. 625-634.
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