Endothelial surface charge of intestinal mucosal capillaries and its modulation by dextran

Ann L. Baldwin, Ning Z. Wu, Daniel L. Stein

    Research output: Contribution to journalArticle

    Abstract

    Capillary endothelial surface charge was investigated by perfusing the intestinal circulation of anesthetized rats in situ with 0.1 or 10 mg/ml native ferritin (NF, pI = 3.8-4.2) or with 0.1 mg/ml cationized ferritin (CF, pI > 10.0) for 5 min, with or without 5% Dextran 40. Ferritin binding was quantified by electron microscopy. All electron micrographs of capillaries perfused with NF showed some NF binding. Mean NF particle densities (particles/μm) were significantly greater at vesicle necks (PDv) than elsewhere on the endothelial surface (PD). Capillaries perfused with CF showed binding in only 60% of the transverse sections examined. The binding was very marked in a large proportion of these vessels. Mean CF particle densities were significantly greater at fenestrae (PDf) than elsewhere. These results demonstrate that mucosal capillaries have a variable negative electrostatic charge on the endothelial surface and support the hypothesis that some vesicle diaphragms act as preferential attractors for anionic macromolecules. Such structures could promote transendothelial vesicular transport of albumin. Dextran significantly decreased PD for NF from 25.4 ± 2.4 (SEM) to 13.1 ± 0.9 and PD, PDv, and PDf for CF from 45.9 ± 4.6 to 31.0 ± 2.7, from 62.1 ± 6.2 to 43.6 ± 5.4, and from 152.9 ± 15.5 to 114.5 ± 8.6, respectively. The above responses result from dextran's weak interaction with the endothelial surface. Dextran reduces access of ferritin molecules to the cell surface by steric hindrance and/or electrostatic shielding of the glycocalyx.

    Original languageEnglish (US)
    Pages (from-to)160-178
    Number of pages19
    JournalMicrovascular Research
    Volume42
    Issue number2
    DOIs
    StatePublished - 1991

    Fingerprint

    Surface charge
    Dextrans
    Ferritins
    Modulation
    Static Electricity
    Electrostatics
    Glycocalyx
    Diaphragms
    Diaphragm
    Macromolecules
    Shielding
    Electron microscopy
    Rats
    Albumins
    Electron Microscopy
    Neck
    Electrons
    Scanning electron microscopy
    Molecules

    ASJC Scopus subject areas

    • Biochemistry
    • Cardiology and Cardiovascular Medicine

    Cite this

    Endothelial surface charge of intestinal mucosal capillaries and its modulation by dextran. / Baldwin, Ann L.; Wu, Ning Z.; Stein, Daniel L.

    In: Microvascular Research, Vol. 42, No. 2, 1991, p. 160-178.

    Research output: Contribution to journalArticle

    Baldwin, Ann L. ; Wu, Ning Z. ; Stein, Daniel L. / Endothelial surface charge of intestinal mucosal capillaries and its modulation by dextran. In: Microvascular Research. 1991 ; Vol. 42, No. 2. pp. 160-178.
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