Endogenous GSK-3/Shaggy regulates bidirectional axonal transport of the amyloid precursor protein

Carole Weaver, Christina Leidel, Lukasz Szpankowski, Nicole M. Farley, George Shubeita, Lawrence S.B. Goldstein

Research output: Contribution to journalArticle

Abstract

Neurons rely on microtubule (MT) motor proteins such as kinesin-1 and dynein to transport essential cargos between the cell body and axon terminus. Defective axonal transport causes abnormal axonal cargo accumulations and is connected to neurodegenerative diseases, including Alzheimer's disease (AD). Glycogen synthase kinase 3 (GSK-3) has been proposed to be a central player in AD and to regulate axonal transport by the MT motor protein kinesin-1. Using genetic, biochemical and biophysical approaches in Drosophila melanogaster, we find that endogenous GSK-3 is a required negative regulator of both kinesin-1-mediated and dynein-mediated axonal transport of the amyloid precursor protein (APP), a key contributor to AD pathology. GSK-3 also regulates transport of an unrelated cargo, embryonic lipid droplets. By measuring the forces motors generate in vivo, we find that GSK-3 regulates transport by altering the activity of kinesin-1 motors but not their binding to the cargo. These findings reveal a new relationship between GSK-3 and APP, and demonstrate that endogenous GSK-3 is an essential in vivo regulator of bidirectional APP transport in axons and lipid droplets in embryos. Furthermore, they point to a new regulatory mechanism in which GSK-3 controls the number of active motors that are moving a cargo.

Original languageEnglish (US)
Pages (from-to)295-308
Number of pages14
JournalTraffic
Volume14
Issue number3
DOIs
StatePublished - Mar 1 2013

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Glycogen Synthase Kinase 3
Axonal Transport
Amyloid beta-Protein Precursor
Kinesin
Microtubule Proteins
Dyneins
Alzheimer Disease
Axons
Neurodegenerative diseases
Lipids
Pathology
Protein Transport
Drosophila melanogaster
Amyloid
Neurodegenerative Diseases
Neurons
Molecular Biology
Proteins
Embryonic Structures
Cells

Keywords

  • Alzheimer's
  • Amyloid precursor protein
  • Axonal transport
  • Dynein
  • GSK-3
  • Kinesin
  • Lipid droplets
  • Optical trap

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Weaver, C., Leidel, C., Szpankowski, L., Farley, N. M., Shubeita, G., & Goldstein, L. S. B. (2013). Endogenous GSK-3/Shaggy regulates bidirectional axonal transport of the amyloid precursor protein. Traffic, 14(3), 295-308. https://doi.org/10.1111/tra.12037

Endogenous GSK-3/Shaggy regulates bidirectional axonal transport of the amyloid precursor protein. / Weaver, Carole; Leidel, Christina; Szpankowski, Lukasz; Farley, Nicole M.; Shubeita, George; Goldstein, Lawrence S.B.

In: Traffic, Vol. 14, No. 3, 01.03.2013, p. 295-308.

Research output: Contribution to journalArticle

Weaver, C, Leidel, C, Szpankowski, L, Farley, NM, Shubeita, G & Goldstein, LSB 2013, 'Endogenous GSK-3/Shaggy regulates bidirectional axonal transport of the amyloid precursor protein', Traffic, vol. 14, no. 3, pp. 295-308. https://doi.org/10.1111/tra.12037
Weaver, Carole ; Leidel, Christina ; Szpankowski, Lukasz ; Farley, Nicole M. ; Shubeita, George ; Goldstein, Lawrence S.B. / Endogenous GSK-3/Shaggy regulates bidirectional axonal transport of the amyloid precursor protein. In: Traffic. 2013 ; Vol. 14, No. 3. pp. 295-308.
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