Embryonic versus blastogenetic development in the compound ascidian Botryllus schlosseri: Insights from Pitx expression patterns

Stefano Tiozzo, Lionel Christiaen, Carole Deyts, Lucia Manni, Jean Stéphane Joly, Paolo Burighel

Research output: Contribution to journalArticle

Abstract

The colonial ascidians reproduce either sexually or asexually, having evolved a rich variety of modes of propagative development. During embryogenesis, the fertilized egg develops into a swimming tadpole larva that subsequently metamorphoses into a sessile oozooid. Clonal individuals (blastozooids), resembling oozooids, are formed from few bud-forming multipotent somatic cells, following a wide range of ways that seem to characterize each family of this class. Here, we compare these two developmental processes in the compound ascidian species Botryllus schlosseri to determine whether similar gene activities are used during embryogenesis/metamorphosis and recruited in the asexual development. We analyzed expression of Pitx, a Paired-related homeobox gene. Pitx genes are key developmental genes in vertebrates, and their expression is reported to be conserved in chordate stomodea and in the establishment of left/right asymmetries. Here, we report full-length cDNA cloning of a B. schlosseri Pitx ortholog (Bs-Pitx) and expression analysis during both embryo/metamorphosis and blastogenesis. During organogenesis of both developmental sequences, Bs-Pitx was detected in identical domains: the stomodeum/neural complex and asymmetrically in the left digestive system. In striking contrast, expression patterns at early stages differ deeply. These observations provide the first evidence for a key developmental gene being deployed in essentially similar ways in two different developmental sequences that eventually give rise to similar zooids.

Original languageEnglish (US)
Pages (from-to)468-478
Number of pages11
JournalDevelopmental Dynamics
Volume232
Issue number2
DOIs
StatePublished - Feb 2005

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Developmental Genes
Urochordata
Embryonic Development
Larva
Chordata
Digestive System
Organogenesis
Zygote
Homeobox Genes
Lymphocyte Activation
Genes
Vertebrates
Organism Cloning
Embryonic Structures
Complementary DNA

Keywords

  • Asexual reproduction
  • Co-option
  • Left/right asymmetry
  • Pituitary
  • Placode
  • Stomodeum

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Embryonic versus blastogenetic development in the compound ascidian Botryllus schlosseri : Insights from Pitx expression patterns. / Tiozzo, Stefano; Christiaen, Lionel; Deyts, Carole; Manni, Lucia; Joly, Jean Stéphane; Burighel, Paolo.

In: Developmental Dynamics, Vol. 232, No. 2, 02.2005, p. 468-478.

Research output: Contribution to journalArticle

Tiozzo, Stefano ; Christiaen, Lionel ; Deyts, Carole ; Manni, Lucia ; Joly, Jean Stéphane ; Burighel, Paolo. / Embryonic versus blastogenetic development in the compound ascidian Botryllus schlosseri : Insights from Pitx expression patterns. In: Developmental Dynamics. 2005 ; Vol. 232, No. 2. pp. 468-478.
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