Embryonic stem cell-based mapping of developmental transcriptional programs

Esteban O. Mazzoni, Shaun Mahony, Michelina Iacovino, Carolyn A. Morrison, George Mountoufaris, Michael Closser, Warren A. Whyte, Richard A. Young, Michael Kyba, David K. Gifford, Hynek Wichterle

Research output: Contribution to journalArticle

Abstract

The study of developmentally regulated transcription factors by chromatin immunoprecipitation and deep sequencing (ChIP-seq) faces two major obstacles: availability of ChIP-grade antibodies and access to sufficient number of cells. We describe versatile genome-wide analysis of transcription-factor binding sites by combining directed differentiation of embryonic stem cells and inducible expression of tagged proteins. We demonstrate its utility by mapping DNA-binding sites of transcription factors involved in motor neuron specification.

Original languageEnglish (US)
Pages (from-to)1056-1060
Number of pages5
JournalNature Methods
Volume8
Issue number12
DOIs
StatePublished - Dec 2011

Fingerprint

Embryonic Stem Cells
Stem cells
Transcription Factors
Binding Sites
High-Throughput Nucleotide Sequencing
Chromatin Immunoprecipitation
Motor Neurons
Neurons
Chromatin
Cell Count
Genes
Availability
Genome
Specifications
Antibodies
DNA
Proteins

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Biochemistry
  • Cell Biology

Cite this

Mazzoni, E. O., Mahony, S., Iacovino, M., Morrison, C. A., Mountoufaris, G., Closser, M., ... Wichterle, H. (2011). Embryonic stem cell-based mapping of developmental transcriptional programs. Nature Methods, 8(12), 1056-1060. https://doi.org/10.1038/nmeth.1775

Embryonic stem cell-based mapping of developmental transcriptional programs. / Mazzoni, Esteban O.; Mahony, Shaun; Iacovino, Michelina; Morrison, Carolyn A.; Mountoufaris, George; Closser, Michael; Whyte, Warren A.; Young, Richard A.; Kyba, Michael; Gifford, David K.; Wichterle, Hynek.

In: Nature Methods, Vol. 8, No. 12, 12.2011, p. 1056-1060.

Research output: Contribution to journalArticle

Mazzoni, EO, Mahony, S, Iacovino, M, Morrison, CA, Mountoufaris, G, Closser, M, Whyte, WA, Young, RA, Kyba, M, Gifford, DK & Wichterle, H 2011, 'Embryonic stem cell-based mapping of developmental transcriptional programs', Nature Methods, vol. 8, no. 12, pp. 1056-1060. https://doi.org/10.1038/nmeth.1775
Mazzoni EO, Mahony S, Iacovino M, Morrison CA, Mountoufaris G, Closser M et al. Embryonic stem cell-based mapping of developmental transcriptional programs. Nature Methods. 2011 Dec;8(12):1056-1060. https://doi.org/10.1038/nmeth.1775
Mazzoni, Esteban O. ; Mahony, Shaun ; Iacovino, Michelina ; Morrison, Carolyn A. ; Mountoufaris, George ; Closser, Michael ; Whyte, Warren A. ; Young, Richard A. ; Kyba, Michael ; Gifford, David K. ; Wichterle, Hynek. / Embryonic stem cell-based mapping of developmental transcriptional programs. In: Nature Methods. 2011 ; Vol. 8, No. 12. pp. 1056-1060.
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