Electrostatic polarization makes a substantial contribution to the free energy of avidin-biotin binding

Yan Tong, Ye Mei, Yong L. Li, Chang G. Ji, John Zhang

Research output: Contribution to journalArticle

Abstract

Avidin-biotin is one of the strongest protein-ligand binding systems, with broad applications in biomedical science. Here we report a quantum-based computational study to help elucidate the mechanism of binding avidin to biotin (BTN1) and its close analogue, 2Â-iminobiotin (BTN2). Our study reveals that electronic polarization of protein plays a critical role in stabilizing the β sheet (Thr113-Arg122) at the binding site and makes a substantial contribution to the free energy of avidin-biotin binding. The current finding is in contradiction to the previous notion that electrostatic interaction has no effect on or makes an unfavorable contribution to the free energy of avidin-biotin binding. Our calculations also show that the difference in binding free energy of avidin to BTN1 and BTN2 is almost entirely due to the contribution of electrostatic interaction resulting from polarization-induced stabilization of a hydrogen bond between avidin and BTN1. The current result provides strong evidence that protein polarization accounts for the electrostatic contribution to binding free energy that was missing in previous studies of avidin-biotin binding.

Original languageEnglish (US)
Pages (from-to)5137-5142
Number of pages6
JournalJournal of the American Chemical Society
Volume132
Issue number14
DOIs
StatePublished - Apr 14 2010

Fingerprint

Avidin
Biotin
Static Electricity
Free energy
Electrostatics
Polarization
Coulomb interactions
Proteins
Binding sites
Hydrogen bonds
Stabilization
Ligands
Protein Binding
Hydrogen
Binding Sites

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Electrostatic polarization makes a substantial contribution to the free energy of avidin-biotin binding. / Tong, Yan; Mei, Ye; Li, Yong L.; Ji, Chang G.; Zhang, John.

In: Journal of the American Chemical Society, Vol. 132, No. 14, 14.04.2010, p. 5137-5142.

Research output: Contribution to journalArticle

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