Electrohydrodynamic Jet 3D Printed Nerve Guide Conduits (NGCs) for peripheral Nerve Injury Repair

Sanjairaj Vijayavenkataraman, Shuo Zhang, Siti Thaharah, Gopu Sriram, Wen Feng Lu, Jerry Ying Hsi Fuh

Research output: Contribution to journalArticle

Abstract

The prevalence of peripheral nerve injuries resulting in loss of motor function, sensory function, or both, is on the rise. Artificial Nerve Guide Conduits (NGCs) are considered an effective alternative treatment for autologous nerve grafts, which is the current gold-standard for treating peripheral nerve injuries. In this study, Polycaprolactone-based three-dimensional porous NGCs are fabricated using Electrohydrodynamic jet 3D printing (EHD-jetting) for the first time. The main advantage of this technique is that all the scaffold properties, namely fibre diameter, pore size, porosity, and fibre alignment, can be controlled by tuning the process parameters. In addition, EHDjetting has the advantages of customizability, repeatability, and scalability. Scaffolds with five different pore sizes (125 to 550 μm) and porosities (65 to 88%) are fabricated and the effect of pore size on the mechanical properties is evaluated. In vitro degradation studies are carried out to investigate the degradation profile of the scaffolds and determine the influence of pore size on the degradation rate and mechanical properties at various degradation time points. Scaffolds with a pore size of 125 ± 15 μm meet the requirements of an optimal NGC structure with a porosity greater than 60%, mechanical properties closer to those of the native peripheral nerves, and an optimal degradation rate matching the nerve regeneration rate post-injury. The in vitro neural differentiation studies also corroborate the same results. Cell proliferation was highest in the scaffolds with a pore size of 125 ± 15 μm assessed by the PrestoBlue assay. The Reverse Transcription-Polymerase Chain Reaction (RT-PCR) results involving the three most important genes concerning neural differentiation, namely β3-tubulin, NF-H, and GAP-43, confirm that the scaffolds with a pore size of 125 ± 15 μm have the highest gene expression of all the other pore sizes and also outperform the electrospun Polycaprolactone (PCL) scaffold. The immunocytochemistry results, expressing the two important nerve proteins β3-tubulin and NF200, showed directional alignment of the neurite growth along the fibre direction in EHD-jet 3D printed scaffolds.

Original languageEnglish (US)
Article number753
JournalPolymers
Volume10
Issue number7
DOIs
StatePublished - Jul 8 2018

Fingerprint

Electrohydrodynamics
Pore size
Scaffolds
Repair
Degradation
Polycaprolactone
Porosity
Tubulin
Mechanical properties
Fibers
GAP-43 Protein
Polymerase chain reaction
Cell proliferation
Transcription
Scaffolds (biology)
Gene expression
Grafts
Gold
Scalability
Printing

Keywords

  • 3D printed scaffolds
  • Electrohydrodynamic jetting
  • Nerve guide conduits
  • Peripheral nerve injury
  • Porous scaffolds
  • Tissue engineering

ASJC Scopus subject areas

  • Chemistry(all)
  • Polymers and Plastics

Cite this

Vijayavenkataraman, S., Zhang, S., Thaharah, S., Sriram, G., Lu, W. F., & Fuh, J. Y. H. (2018). Electrohydrodynamic Jet 3D Printed Nerve Guide Conduits (NGCs) for peripheral Nerve Injury Repair. Polymers, 10(7), [753]. https://doi.org/10.3390/polym10070753

Electrohydrodynamic Jet 3D Printed Nerve Guide Conduits (NGCs) for peripheral Nerve Injury Repair. / Vijayavenkataraman, Sanjairaj; Zhang, Shuo; Thaharah, Siti; Sriram, Gopu; Lu, Wen Feng; Fuh, Jerry Ying Hsi.

In: Polymers, Vol. 10, No. 7, 753, 08.07.2018.

Research output: Contribution to journalArticle

Vijayavenkataraman, S, Zhang, S, Thaharah, S, Sriram, G, Lu, WF & Fuh, JYH 2018, 'Electrohydrodynamic Jet 3D Printed Nerve Guide Conduits (NGCs) for peripheral Nerve Injury Repair', Polymers, vol. 10, no. 7, 753. https://doi.org/10.3390/polym10070753
Vijayavenkataraman S, Zhang S, Thaharah S, Sriram G, Lu WF, Fuh JYH. Electrohydrodynamic Jet 3D Printed Nerve Guide Conduits (NGCs) for peripheral Nerve Injury Repair. Polymers. 2018 Jul 8;10(7). 753. https://doi.org/10.3390/polym10070753
Vijayavenkataraman, Sanjairaj ; Zhang, Shuo ; Thaharah, Siti ; Sriram, Gopu ; Lu, Wen Feng ; Fuh, Jerry Ying Hsi. / Electrohydrodynamic Jet 3D Printed Nerve Guide Conduits (NGCs) for peripheral Nerve Injury Repair. In: Polymers. 2018 ; Vol. 10, No. 7.
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abstract = "The prevalence of peripheral nerve injuries resulting in loss of motor function, sensory function, or both, is on the rise. Artificial Nerve Guide Conduits (NGCs) are considered an effective alternative treatment for autologous nerve grafts, which is the current gold-standard for treating peripheral nerve injuries. In this study, Polycaprolactone-based three-dimensional porous NGCs are fabricated using Electrohydrodynamic jet 3D printing (EHD-jetting) for the first time. The main advantage of this technique is that all the scaffold properties, namely fibre diameter, pore size, porosity, and fibre alignment, can be controlled by tuning the process parameters. In addition, EHDjetting has the advantages of customizability, repeatability, and scalability. Scaffolds with five different pore sizes (125 to 550 μm) and porosities (65 to 88{\%}) are fabricated and the effect of pore size on the mechanical properties is evaluated. In vitro degradation studies are carried out to investigate the degradation profile of the scaffolds and determine the influence of pore size on the degradation rate and mechanical properties at various degradation time points. Scaffolds with a pore size of 125 ± 15 μm meet the requirements of an optimal NGC structure with a porosity greater than 60{\%}, mechanical properties closer to those of the native peripheral nerves, and an optimal degradation rate matching the nerve regeneration rate post-injury. The in vitro neural differentiation studies also corroborate the same results. Cell proliferation was highest in the scaffolds with a pore size of 125 ± 15 μm assessed by the PrestoBlue assay. The Reverse Transcription-Polymerase Chain Reaction (RT-PCR) results involving the three most important genes concerning neural differentiation, namely β3-tubulin, NF-H, and GAP-43, confirm that the scaffolds with a pore size of 125 ± 15 μm have the highest gene expression of all the other pore sizes and also outperform the electrospun Polycaprolactone (PCL) scaffold. The immunocytochemistry results, expressing the two important nerve proteins β3-tubulin and NF200, showed directional alignment of the neurite growth along the fibre direction in EHD-jet 3D printed scaffolds.",
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