Electrohydrodynamic generation and delivery of monodisperse picoliter droplets using a poly(dimethylsiloxane) microchip

Jae Kim Sung, Rafael Song, Paul L. Skipper, Jongyoon Han

    Research output: Contribution to journalArticle

    Abstract

    We developed a drop-on-demand microdroplet generator for the discrete dispensing of biosamples into a bio-analytical unit. This disposable PDMS microfluidic device can generate monodisperse droplets of picoliter volume directly out of a plane sidewall of the microfluidic chip by an electrohydrodynamic mechanism. The droplet generation was accomplished without using either an inserted capillary or a monolithically built-in tip. The minimum droplet volume was ∼4 pL, and the droplet generation was repeatable and stable for at least 30 min, with a typical variation of less than 2.0% of drop size. The Taylor cone, which is usually observed in electrospray, was suppressed by controlling the surface wetting property of the PDMS device as well as the surface tension of the sample liquids. A modification of the channel geometry right before the opening of the microchannel also enhanced the continuous droplet generation without applying any external pumping. A simple numerical simulation of the droplet generation verified the importance of controlling the surface wetting conditions for the droplet formation. Our microdroplet generator can be effectively applied to a direct interface of a microfluidic chip to a biosensing unit, such as AMS, MALDI-MS or protein microarray-type biochips.

    Original languageEnglish (US)
    Pages (from-to)8011-8019
    Number of pages9
    JournalAnalytical Chemistry
    Volume78
    Issue number23
    DOIs
    StatePublished - Dec 1 2006

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    Electrohydrodynamics
    Microfluidics
    Wetting
    baysilon
    Biochips
    Microarrays
    Microchannels
    Surface tension
    Cones
    Geometry

    ASJC Scopus subject areas

    • Analytical Chemistry

    Cite this

    Electrohydrodynamic generation and delivery of monodisperse picoliter droplets using a poly(dimethylsiloxane) microchip. / Sung, Jae Kim; Song, Rafael; Skipper, Paul L.; Han, Jongyoon.

    In: Analytical Chemistry, Vol. 78, No. 23, 01.12.2006, p. 8011-8019.

    Research output: Contribution to journalArticle

    Sung, Jae Kim ; Song, Rafael ; Skipper, Paul L. ; Han, Jongyoon. / Electrohydrodynamic generation and delivery of monodisperse picoliter droplets using a poly(dimethylsiloxane) microchip. In: Analytical Chemistry. 2006 ; Vol. 78, No. 23. pp. 8011-8019.
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