Electrochemical studies of morpholino-DNA surface hybridization

R. O'Connor, N. Tercero, W. Qiao, R. Levicky

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Surface hybridization, in which nucleic acids from solution bind to complementary "probe" strands immobilized on a solid support, is widely used to analyze composition of nucleic acid mixtures. Most often, detection is accomplished with fluorescent techniques whose sensitivity can be extended down to individual molecules. Applications, however, benefit as much if not more from convenience, accuracy, and affordability of the diagnostic test. By eliminating the need for fluorescent labeling and more complex sample workup, label-free electrochemical assays have significant advantages provided transduction remains sufficiently sensitive for applications. To this end, we have been exploring morpholinos, which are uncharged DNA analogues, as the immobilized probe species in surface hybridization assays based on measurement of interfacial capacitance. Through comparison of experimental trends with those predicted from basic physical models, the origins of diagnostic contrast in capacitive sensing are reviewed for assays based on morpholino as well as on DNA probes.

Original languageEnglish (US)
Title of host publicationBioelectronics, Biointerfaces, and Biomedical Applications 4
Pages99-110
Number of pages12
Edition7
DOIs
StatePublished - Aug 1 2011
EventBioelectronics, Biointerfaces, and Biomedical Applications 4 - 219th ECS Meeting - Montreal, QC, Canada
Duration: May 1 2011May 6 2011

Publication series

NameECS Transactions
Number7
Volume35
ISSN (Print)1938-5862
ISSN (Electronic)1938-6737

Other

OtherBioelectronics, Biointerfaces, and Biomedical Applications 4 - 219th ECS Meeting
CountryCanada
CityMontreal, QC
Period5/1/115/6/11

    Fingerprint

ASJC Scopus subject areas

  • Engineering(all)

Cite this

O'Connor, R., Tercero, N., Qiao, W., & Levicky, R. (2011). Electrochemical studies of morpholino-DNA surface hybridization. In Bioelectronics, Biointerfaces, and Biomedical Applications 4 (7 ed., pp. 99-110). (ECS Transactions; Vol. 35, No. 7). https://doi.org/10.1149/1.3571981