Electrocautery-induced localized colonic injury elicits increased levels of pro-inflammatory cytokines in small bowel and decreases jejunal alanine absorption

Kassem Barada, Fadi H. Mourad, Bakiza Noutsi, Nayef E. Saadé

Research output: Contribution to journalArticle

Abstract

Background: Colitis is associated with functional abnormalities in proximal non-inflamed gut areas, but animal models to study small bowel dysfunction in colitis have limitations. This study aims to determine small intestinal alanine absorption and cytokine expression in a novel model of colonic ulceration induced by electro-cautery. Methods: A descending colon ulcer was induced in rats by a bipolar electro-cautery probe. Ulcer score was determined using Satoh's criteria. Jejunal alanine absorption was measured immediately and at different time intervals post ulcer induction. Levels of interleukin-1 (IL-1), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) protein and m-RNA were determined in mucosal scrapings obtained from the colon, duodenum, jejunum and ileum at various time intervals after colonic ulcer induction. Results: The mean ulcer score was 3 up to 48. h, followed by healing by 96. h post ulcer induction. Small bowel histology was normal throughout. Jejunal alanine absorption was reduced by 12-34% immediately and up to 72. h after cautery and returned to normal at 96. h. IL-1 and TNF-α mRNA increased significantly in the colon, duodenum, jejunum and ileum 3. h post electro-cautery and returned to normal at 48. h, while that of IL-6 increased significantly at 48. h post ulcer induction. Similarly, IL-1, IL-6 and TNF-α protein levels increased in the duodenum, jejunum, ileum and colon up to 48. h post ulcer induction. Conclusions: Electrically induced localized colonic injury increased production of pro-inflammatory cytokines in non-inflamed segments of the small intestine and was associated with derangements of jejunal absorptive function.

Original languageEnglish (US)
Pages (from-to)109-118
Number of pages10
JournalCytokine
Volume71
Issue number1
DOIs
StatePublished - Jan 1 2015

Fingerprint

Electrocoagulation
Interleukin-1
Alanine
Ulcer
Interleukin-6
Tumor Necrosis Factor-alpha
Cytokines
Cautery
Wounds and Injuries
Histology
Jejunum
Ileum
Duodenum
Colon
Rats
Animals
Proteins
Colitis
RNA
Messenger RNA

Keywords

  • Alanine absorption
  • Colitis
  • Cytokines
  • Electro-cautery

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Hematology
  • Molecular Biology

Cite this

Electrocautery-induced localized colonic injury elicits increased levels of pro-inflammatory cytokines in small bowel and decreases jejunal alanine absorption. / Barada, Kassem; Mourad, Fadi H.; Noutsi, Bakiza; Saadé, Nayef E.

In: Cytokine, Vol. 71, No. 1, 01.01.2015, p. 109-118.

Research output: Contribution to journalArticle

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abstract = "Background: Colitis is associated with functional abnormalities in proximal non-inflamed gut areas, but animal models to study small bowel dysfunction in colitis have limitations. This study aims to determine small intestinal alanine absorption and cytokine expression in a novel model of colonic ulceration induced by electro-cautery. Methods: A descending colon ulcer was induced in rats by a bipolar electro-cautery probe. Ulcer score was determined using Satoh's criteria. Jejunal alanine absorption was measured immediately and at different time intervals post ulcer induction. Levels of interleukin-1 (IL-1), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) protein and m-RNA were determined in mucosal scrapings obtained from the colon, duodenum, jejunum and ileum at various time intervals after colonic ulcer induction. Results: The mean ulcer score was 3 up to 48. h, followed by healing by 96. h post ulcer induction. Small bowel histology was normal throughout. Jejunal alanine absorption was reduced by 12-34{\%} immediately and up to 72. h after cautery and returned to normal at 96. h. IL-1 and TNF-α mRNA increased significantly in the colon, duodenum, jejunum and ileum 3. h post electro-cautery and returned to normal at 48. h, while that of IL-6 increased significantly at 48. h post ulcer induction. Similarly, IL-1, IL-6 and TNF-α protein levels increased in the duodenum, jejunum, ileum and colon up to 48. h post ulcer induction. Conclusions: Electrically induced localized colonic injury increased production of pro-inflammatory cytokines in non-inflamed segments of the small intestine and was associated with derangements of jejunal absorptive function.",
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N2 - Background: Colitis is associated with functional abnormalities in proximal non-inflamed gut areas, but animal models to study small bowel dysfunction in colitis have limitations. This study aims to determine small intestinal alanine absorption and cytokine expression in a novel model of colonic ulceration induced by electro-cautery. Methods: A descending colon ulcer was induced in rats by a bipolar electro-cautery probe. Ulcer score was determined using Satoh's criteria. Jejunal alanine absorption was measured immediately and at different time intervals post ulcer induction. Levels of interleukin-1 (IL-1), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) protein and m-RNA were determined in mucosal scrapings obtained from the colon, duodenum, jejunum and ileum at various time intervals after colonic ulcer induction. Results: The mean ulcer score was 3 up to 48. h, followed by healing by 96. h post ulcer induction. Small bowel histology was normal throughout. Jejunal alanine absorption was reduced by 12-34% immediately and up to 72. h after cautery and returned to normal at 96. h. IL-1 and TNF-α mRNA increased significantly in the colon, duodenum, jejunum and ileum 3. h post electro-cautery and returned to normal at 48. h, while that of IL-6 increased significantly at 48. h post ulcer induction. Similarly, IL-1, IL-6 and TNF-α protein levels increased in the duodenum, jejunum, ileum and colon up to 48. h post ulcer induction. Conclusions: Electrically induced localized colonic injury increased production of pro-inflammatory cytokines in non-inflamed segments of the small intestine and was associated with derangements of jejunal absorptive function.

AB - Background: Colitis is associated with functional abnormalities in proximal non-inflamed gut areas, but animal models to study small bowel dysfunction in colitis have limitations. This study aims to determine small intestinal alanine absorption and cytokine expression in a novel model of colonic ulceration induced by electro-cautery. Methods: A descending colon ulcer was induced in rats by a bipolar electro-cautery probe. Ulcer score was determined using Satoh's criteria. Jejunal alanine absorption was measured immediately and at different time intervals post ulcer induction. Levels of interleukin-1 (IL-1), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) protein and m-RNA were determined in mucosal scrapings obtained from the colon, duodenum, jejunum and ileum at various time intervals after colonic ulcer induction. Results: The mean ulcer score was 3 up to 48. h, followed by healing by 96. h post ulcer induction. Small bowel histology was normal throughout. Jejunal alanine absorption was reduced by 12-34% immediately and up to 72. h after cautery and returned to normal at 96. h. IL-1 and TNF-α mRNA increased significantly in the colon, duodenum, jejunum and ileum 3. h post electro-cautery and returned to normal at 48. h, while that of IL-6 increased significantly at 48. h post ulcer induction. Similarly, IL-1, IL-6 and TNF-α protein levels increased in the duodenum, jejunum, ileum and colon up to 48. h post ulcer induction. Conclusions: Electrically induced localized colonic injury increased production of pro-inflammatory cytokines in non-inflamed segments of the small intestine and was associated with derangements of jejunal absorptive function.

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KW - Cytokines

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