Electrical silencing of Drosophila pacemaker neurons stops the free-running circadian clock

Michael N. Nitabach, Justin Blau, Todd C. Holmes

Research output: Contribution to journalArticle

Abstract

Electrical silencing of Drosophila circadian pacemaker neurons through targeted expression of K+ channels causes severe deficits in free-running circadian locomotor rhythmicity in complete darkness. Pacemaker electrical silencing also stops the free-running oscillation of PERIOD (PER) and TIMELESS (TIM) proteins that constitutes the core of the cell-autonomous molecular clock. In contrast, electrical silencing fails to abolish PER and TIM oscillation in light-dark cycles, although it does impair rhythmic behavior. On the basis of these findings, we propose that electrical activity is an essential element of the free-running molecular clock of pacemaker neurons along with the transcription factors and regulatory enzymes that have been previously identified as required for clock function.

Original languageEnglish (US)
Pages (from-to)485-495
Number of pages11
JournalCell
Volume109
Issue number4
DOIs
StatePublished - May 17 2002

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Pacemakers
Circadian Clocks
Drosophila
Neurons
Clocks
Darkness
Photoperiod
Periodicity
Running
Transcription Factors
Enzymes
Proteins

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Electrical silencing of Drosophila pacemaker neurons stops the free-running circadian clock. / Nitabach, Michael N.; Blau, Justin; Holmes, Todd C.

In: Cell, Vol. 109, No. 4, 17.05.2002, p. 485-495.

Research output: Contribution to journalArticle

Nitabach, Michael N. ; Blau, Justin ; Holmes, Todd C. / Electrical silencing of Drosophila pacemaker neurons stops the free-running circadian clock. In: Cell. 2002 ; Vol. 109, No. 4. pp. 485-495.
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