Abstract
Electrical silencing of Drosophila circadian pacemaker neurons through targeted expression of K+ channels causes severe deficits in free-running circadian locomotor rhythmicity in complete darkness. Pacemaker electrical silencing also stops the free-running oscillation of PERIOD (PER) and TIMELESS (TIM) proteins that constitutes the core of the cell-autonomous molecular clock. In contrast, electrical silencing fails to abolish PER and TIM oscillation in light-dark cycles, although it does impair rhythmic behavior. On the basis of these findings, we propose that electrical activity is an essential element of the free-running molecular clock of pacemaker neurons along with the transcription factors and regulatory enzymes that have been previously identified as required for clock function.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 485-495 |
| Number of pages | 11 |
| Journal | Cell |
| Volume | 109 |
| Issue number | 4 |
| DOIs | |
| State | Published - May 17 2002 |
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ASJC Scopus subject areas
- Cell Biology
- Molecular Biology
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Electrical silencing of Drosophila pacemaker neurons stops the free-running circadian clock. / Nitabach, Michael N.; Blau, Justin; Holmes, Todd C.
In: Cell, Vol. 109, No. 4, 17.05.2002, p. 485-495.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Electrical silencing of Drosophila pacemaker neurons stops the free-running circadian clock
AU - Nitabach, Michael N.
AU - Blau, Justin
AU - Holmes, Todd C.
PY - 2002/5/17
Y1 - 2002/5/17
N2 - Electrical silencing of Drosophila circadian pacemaker neurons through targeted expression of K+ channels causes severe deficits in free-running circadian locomotor rhythmicity in complete darkness. Pacemaker electrical silencing also stops the free-running oscillation of PERIOD (PER) and TIMELESS (TIM) proteins that constitutes the core of the cell-autonomous molecular clock. In contrast, electrical silencing fails to abolish PER and TIM oscillation in light-dark cycles, although it does impair rhythmic behavior. On the basis of these findings, we propose that electrical activity is an essential element of the free-running molecular clock of pacemaker neurons along with the transcription factors and regulatory enzymes that have been previously identified as required for clock function.
AB - Electrical silencing of Drosophila circadian pacemaker neurons through targeted expression of K+ channels causes severe deficits in free-running circadian locomotor rhythmicity in complete darkness. Pacemaker electrical silencing also stops the free-running oscillation of PERIOD (PER) and TIMELESS (TIM) proteins that constitutes the core of the cell-autonomous molecular clock. In contrast, electrical silencing fails to abolish PER and TIM oscillation in light-dark cycles, although it does impair rhythmic behavior. On the basis of these findings, we propose that electrical activity is an essential element of the free-running molecular clock of pacemaker neurons along with the transcription factors and regulatory enzymes that have been previously identified as required for clock function.
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U2 - 10.1016/S0092-8674(02)00737-7
DO - 10.1016/S0092-8674(02)00737-7
M3 - Article
VL - 109
SP - 485
EP - 495
JO - Cell
JF - Cell
SN - 0092-8674
IS - 4
ER -