EGG-4 and EGG-5 Link Events of the Oocyte-to-Embryo Transition with Meiotic Progression in C. elegans

Jean M. Parry, Nathalie V. Velarde, Ariel J. Lefkovith, Matthew H. Zegarek, Julie S. Hang, Jonathan Ohm, Richard Klancer, Rika Maruyama, Marina K. Druzhinina, Barth D. Grant, Fabio Piano, Andrew Singson

Research output: Contribution to journalArticle

Abstract

The molecular underpinnings of the oocyte-to-embryo transition are poorly understood. Here we show that two protein tyrosine phosphatase-like (PTPL) family proteins, EGG-4 and EGG-5, are required for key events of the oocyte-to-embryo transition in Caenorhabditis elegans. The predicted EGG-4 and EGG-5 amino acid sequences are 99% identical and their functions are redundant. In embryos lacking EGG-4 and EGG-5, we observe defects in meiosis, polar body formation, the block to polyspermy, F-actin dynamics, and eggshell deposition. During oogenesis, EGG-4 and EGG-5 assemble at the oocyte cortex with the previously identified regulators or effectors of the oocyte-to-embryo transition EGG-3, CHS-1, and MBK-2 [1, 2]. All of these molecules share a complex interdependence with regards to their dynamics and subcellular localization. Shortly after fertilization, EGG-4 and EGG-5 are required to properly coordinate a redistribution of CHS-1 and EGG-3 away from the cortex during meiotic anaphase I. Therefore, EGG-4 and EGG-5 are not only required for critical events of the oocyte-to-embryo transition but also link the dynamics of the regulatory machinery with the advancing cell cycle.

Original languageEnglish (US)
Pages (from-to)1752-1757
Number of pages6
JournalCurrent Biology
Volume19
Issue number20
DOIs
StatePublished - Nov 3 2009

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Oocytes
Ovum
embryo (animal)
oocytes
Embryonic Structures
Protein Tyrosine Phosphatases
cortex
Machinery
Actins
polyspermy
protein-tyrosine-phosphatase
Cells
anaphase
Amino Acids
oogenesis
Caenorhabditis elegans
fertilization (reproduction)
Defects
egg shell
Molecules

Keywords

  • CELLCYCLE
  • DEVBIO

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Parry, J. M., Velarde, N. V., Lefkovith, A. J., Zegarek, M. H., Hang, J. S., Ohm, J., ... Singson, A. (2009). EGG-4 and EGG-5 Link Events of the Oocyte-to-Embryo Transition with Meiotic Progression in C. elegans. Current Biology, 19(20), 1752-1757. https://doi.org/10.1016/j.cub.2009.09.015

EGG-4 and EGG-5 Link Events of the Oocyte-to-Embryo Transition with Meiotic Progression in C. elegans. / Parry, Jean M.; Velarde, Nathalie V.; Lefkovith, Ariel J.; Zegarek, Matthew H.; Hang, Julie S.; Ohm, Jonathan; Klancer, Richard; Maruyama, Rika; Druzhinina, Marina K.; Grant, Barth D.; Piano, Fabio; Singson, Andrew.

In: Current Biology, Vol. 19, No. 20, 03.11.2009, p. 1752-1757.

Research output: Contribution to journalArticle

Parry, JM, Velarde, NV, Lefkovith, AJ, Zegarek, MH, Hang, JS, Ohm, J, Klancer, R, Maruyama, R, Druzhinina, MK, Grant, BD, Piano, F & Singson, A 2009, 'EGG-4 and EGG-5 Link Events of the Oocyte-to-Embryo Transition with Meiotic Progression in C. elegans', Current Biology, vol. 19, no. 20, pp. 1752-1757. https://doi.org/10.1016/j.cub.2009.09.015
Parry, Jean M. ; Velarde, Nathalie V. ; Lefkovith, Ariel J. ; Zegarek, Matthew H. ; Hang, Julie S. ; Ohm, Jonathan ; Klancer, Richard ; Maruyama, Rika ; Druzhinina, Marina K. ; Grant, Barth D. ; Piano, Fabio ; Singson, Andrew. / EGG-4 and EGG-5 Link Events of the Oocyte-to-Embryo Transition with Meiotic Progression in C. elegans. In: Current Biology. 2009 ; Vol. 19, No. 20. pp. 1752-1757.
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