Efficient in vivo gene delivery using modified Tat peptide with cationic lipids

Seiichi Yamano, Jisen Dai, Shigeru Hanatani, Ken Haku, Takuto Yamanaka, Mika Ishioka, Tadahiro Takayama, Amr Moursi

Research output: Contribution to journalArticle

Abstract

A combination of modified HIV-1 Tat (mTat) peptide and cationic lipids, FuGENE HD (FH), dramatically enhanced transfection efficiency across a range of cell lines when compared to mTat or FH alone (Biomaterials 35:1705-1715 2014). The efficiency of this Tat peptide combination was significantly higher than many commercial non-viral vectors. In this present study, we tested the feasibility of this non-viral vector, mTat/FH, in vivo using plasmid DNA encoding a luciferase gene. The results of the in vivo studies showed that animals administered mTat/FH/DNA intramuscularly had significantly higher and longer luciferase expression (≈7 months) than those with mTat/DNA, FH/DNA, or DNA alone. Histological evaluation showed little immune response in the muscles, livers, and kidneys of mice administered with the mTat/FH. The combination of mTat with FH could significantly improve transfection efficiency, expanding the potential use of non-viral gene vectors in vivo.

Original languageEnglish (US)
Pages (from-to)1447-1452
Number of pages6
JournalBiotechnology Letters
Volume36
Issue number7
DOIs
StatePublished - 2014

Fingerprint

Lipids
Peptides
HIV-1
DNA
Genes
Luciferases
Transfection
Biocompatible Materials
Biomaterials
Liver
Muscle
Animals
Plasmids
Cells
Kidney
Cell Line
Muscles

Keywords

  • Cationic lipids
  • Cell-penetrating peptides
  • Gene delivery
  • Non-viral vector
  • Plasmid DNA
  • Tat peptide
  • Transfection efficiency

ASJC Scopus subject areas

  • Biotechnology

Cite this

Efficient in vivo gene delivery using modified Tat peptide with cationic lipids. / Yamano, Seiichi; Dai, Jisen; Hanatani, Shigeru; Haku, Ken; Yamanaka, Takuto; Ishioka, Mika; Takayama, Tadahiro; Moursi, Amr.

In: Biotechnology Letters, Vol. 36, No. 7, 2014, p. 1447-1452.

Research output: Contribution to journalArticle

Yamano, S, Dai, J, Hanatani, S, Haku, K, Yamanaka, T, Ishioka, M, Takayama, T & Moursi, A 2014, 'Efficient in vivo gene delivery using modified Tat peptide with cationic lipids', Biotechnology Letters, vol. 36, no. 7, pp. 1447-1452. https://doi.org/10.1007/s10529-014-1497-2
Yamano, Seiichi ; Dai, Jisen ; Hanatani, Shigeru ; Haku, Ken ; Yamanaka, Takuto ; Ishioka, Mika ; Takayama, Tadahiro ; Moursi, Amr. / Efficient in vivo gene delivery using modified Tat peptide with cationic lipids. In: Biotechnology Letters. 2014 ; Vol. 36, No. 7. pp. 1447-1452.
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AU - Dai, Jisen

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AU - Yamanaka, Takuto

AU - Ishioka, Mika

AU - Takayama, Tadahiro

AU - Moursi, Amr

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