Effects of Saharan dust on the microbial community during a large in situ mesocosm experiment in the NW Mediterranean Sea

M. Laghdass, S. Blain, M. Besseling, P. Catala, Cecile Guieu, I. Obernosterer

    Research output: Contribution to journalArticle

    Abstract

    The response of the microbial community to Saharan dust deposition was investigated in 6 large mesocosms (52 m3) deployed at an oligotrophic coastal site in the NW Mediterranean Sea in June 2008 (DUNE project). The mesocosms represented well the environmental conditions observed at the study site during the 8 d experimental period, and the triplicate mesocosms exhibited high reproducibility for each treatment. Dust deposition resulted in an increase in chlorophyll a concentration (0.22 ± 0.03 μg l-1), as compared to that in the control treatments (0.12 ± 0.01 μg l -1), but no treatment effect was observed for bacterial heterotrophic abundance at 5 m depth. Results from the fingerprinting technique CE-SSCP indicate a temporal evolution of the structure of the total (16S rRNA gene) and active (16S rRNA transcripts) bacterial community, and Saharan dust deposition had a noticeable structuring effect on the active bacterial community. Combining results from 16S rRNA gene clone libraries and CE-SSCP indicates that the relative contribution of Alteromonas macleodii to the active bacterial community was enhanced 2-fold following dust addition. The 2 operational taxonomic units (OTUs) Thiothrix and Alteromonas, belonging to Gammaproteobacteria, and the Bacteroidetes OTU NS5-1 were specific to the clone libraries from the dust-amended mesocosms or more abundant in these than in the control ones. CARD-FISH analyses, however, indicate that these OTUs had overall low abundances (1 to 5% of total DAPI-counts). Despite the pronounced temporal trend observed during the experimental period, dust deposition had a small, but noticeable structuring effect on the heterotrophic bacterial community that was detectable only at the OTU level at 99% similarity of the 16S rRNA gene.

    Original languageEnglish (US)
    Pages (from-to)201-213
    Number of pages13
    JournalAquatic Microbial Ecology
    Volume62
    Issue number2
    DOIs
    StatePublished - Jan 19 2011

    Fingerprint

    mesocosm
    Mediterranean Sea
    dust
    microbial communities
    microbial community
    bacterial communities
    ribosomal RNA
    experiment
    gene
    clone
    Thiothrix
    Alteromonas macleodii
    Alteromonas
    clones
    genes
    gamma-Proteobacteria
    temporal evolution
    reproducibility
    in situ
    effect

    Keywords

    • Alteromonas macleodii
    • CARD-FISH
    • CE-SSCP
    • Clone libraries
    • In situ mesocosms
    • NW Mediterranean Sea
    • Saharan dust deposition

    ASJC Scopus subject areas

    • Ecology, Evolution, Behavior and Systematics
    • Aquatic Science

    Cite this

    Effects of Saharan dust on the microbial community during a large in situ mesocosm experiment in the NW Mediterranean Sea. / Laghdass, M.; Blain, S.; Besseling, M.; Catala, P.; Guieu, Cecile; Obernosterer, I.

    In: Aquatic Microbial Ecology, Vol. 62, No. 2, 19.01.2011, p. 201-213.

    Research output: Contribution to journalArticle

    Laghdass, M. ; Blain, S. ; Besseling, M. ; Catala, P. ; Guieu, Cecile ; Obernosterer, I. / Effects of Saharan dust on the microbial community during a large in situ mesocosm experiment in the NW Mediterranean Sea. In: Aquatic Microbial Ecology. 2011 ; Vol. 62, No. 2. pp. 201-213.
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    abstract = "The response of the microbial community to Saharan dust deposition was investigated in 6 large mesocosms (52 m3) deployed at an oligotrophic coastal site in the NW Mediterranean Sea in June 2008 (DUNE project). The mesocosms represented well the environmental conditions observed at the study site during the 8 d experimental period, and the triplicate mesocosms exhibited high reproducibility for each treatment. Dust deposition resulted in an increase in chlorophyll a concentration (0.22 ± 0.03 μg l-1), as compared to that in the control treatments (0.12 ± 0.01 μg l -1), but no treatment effect was observed for bacterial heterotrophic abundance at 5 m depth. Results from the fingerprinting technique CE-SSCP indicate a temporal evolution of the structure of the total (16S rRNA gene) and active (16S rRNA transcripts) bacterial community, and Saharan dust deposition had a noticeable structuring effect on the active bacterial community. Combining results from 16S rRNA gene clone libraries and CE-SSCP indicates that the relative contribution of Alteromonas macleodii to the active bacterial community was enhanced 2-fold following dust addition. The 2 operational taxonomic units (OTUs) Thiothrix and Alteromonas, belonging to Gammaproteobacteria, and the Bacteroidetes OTU NS5-1 were specific to the clone libraries from the dust-amended mesocosms or more abundant in these than in the control ones. CARD-FISH analyses, however, indicate that these OTUs had overall low abundances (1 to 5{\%} of total DAPI-counts). Despite the pronounced temporal trend observed during the experimental period, dust deposition had a small, but noticeable structuring effect on the heterotrophic bacterial community that was detectable only at the OTU level at 99{\%} similarity of the 16S rRNA gene.",
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    AU - Guieu, Cecile

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