Effects of platelet-derived growth factor-AA and -BB on embryonic cardiac development

Robert L. Price, Stephen T. Haley, Tara A. Bullard, Edie C. Goldsmith, David G. Simpson, Thomas E. Thielen, Michael J. Yost, Louis Terracio

Research output: Contribution to journalArticle

Abstract

Several studies have shown that disruption of the normal expression patterns of platelet-derived growth factor (PDGF) ligands and receptors during development results in gross cardiac defects and embryonic or neonatal death. However, little is known about the specific role that PDGF plays in the differentiation of cardiac myocytes. In experiments complementing studies that utilized naturally-occurring Patch mice lacking the PDGFr α, or knockout animals lacking a PDGF ligand or receptor, we used rat and mouse whole-embryo culture (WEC) techniques to increase the exposure of embryos to the PDGF-AA or -BB ligands. Following a 48-hr culture period, we analyzed heart growth and cardiac myocyte differentiation. Exposure of rat embryos to 50 ng/ml of PDGF-AA resulted in a 42% increase in total protein levels in the heart, but did not result in a significant increase in heart growth, as determined by measurements of the atrioventricular length and the left ventricular length and width. Exposure of embryos to 50 ng/ml of PDGF-BB resulted in a 77% increase in total protein levels and a significant (P < 0.05) 8-15% increase in the measured heart parameters. Although a comparison of control and PDGF-AA-treated embryos showed no increase in the overall size of the heart, confocal microscopy showed an increase in the size and number of myofibrillar bundles in the developing myocardium. In addition, transmission electron microscopy (TEM) revealed an increase in the presence of sarcomeres, indicating that myofibrils were more highly differentiated in these areas of the treated embryos. In PDGF-BB-treated embryos, the compact zone of the myocardium was thicker and, as shown by confocal microscopy and TEM, Lactin and well-developed sarcomeres were more prevalent, indicating that the myofibrils were more differentiated in the treated embryos than in the control embryos. These studies indicate that increased exposure of embryonic hearts to PDGF-AA or -BB increases the rate of myocardial development.

Original languageEnglish (US)
Pages (from-to)424-433
Number of pages10
JournalAnatomical Record - Part A Discoveries in Molecular, Cellular, and Evolutionary Biology
Volume272
Issue number1
StatePublished - May 1 2003

Fingerprint

platelet-derived growth factor
embryonic development
Embryonic Development
Platelet-Derived Growth Factor
Embryonic Structures
embryo (animal)
embryo
heart
Sarcomeres
Myofibrils
sarcomeres
Ligands
myofibrils
Transmission Electron Microscopy
ligand
Cardiac Myocytes
Confocal Microscopy
myocardium
Embryo Culture Techniques
Myocardium

Keywords

  • Cardiac development
  • Confocal microscopy
  • Electron microscopy
  • Myofibrillogenesis
  • Platelet-derived growth factor

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Anatomy

Cite this

Effects of platelet-derived growth factor-AA and -BB on embryonic cardiac development. / Price, Robert L.; Haley, Stephen T.; Bullard, Tara A.; Goldsmith, Edie C.; Simpson, David G.; Thielen, Thomas E.; Yost, Michael J.; Terracio, Louis.

In: Anatomical Record - Part A Discoveries in Molecular, Cellular, and Evolutionary Biology, Vol. 272, No. 1, 01.05.2003, p. 424-433.

Research output: Contribution to journalArticle

Price, RL, Haley, ST, Bullard, TA, Goldsmith, EC, Simpson, DG, Thielen, TE, Yost, MJ & Terracio, L 2003, 'Effects of platelet-derived growth factor-AA and -BB on embryonic cardiac development', Anatomical Record - Part A Discoveries in Molecular, Cellular, and Evolutionary Biology, vol. 272, no. 1, pp. 424-433.
Price, Robert L. ; Haley, Stephen T. ; Bullard, Tara A. ; Goldsmith, Edie C. ; Simpson, David G. ; Thielen, Thomas E. ; Yost, Michael J. ; Terracio, Louis. / Effects of platelet-derived growth factor-AA and -BB on embryonic cardiac development. In: Anatomical Record - Part A Discoveries in Molecular, Cellular, and Evolutionary Biology. 2003 ; Vol. 272, No. 1. pp. 424-433.
@article{326c90945c56408183510f9ccaf1c949,
title = "Effects of platelet-derived growth factor-AA and -BB on embryonic cardiac development",
abstract = "Several studies have shown that disruption of the normal expression patterns of platelet-derived growth factor (PDGF) ligands and receptors during development results in gross cardiac defects and embryonic or neonatal death. However, little is known about the specific role that PDGF plays in the differentiation of cardiac myocytes. In experiments complementing studies that utilized naturally-occurring Patch mice lacking the PDGFr α, or knockout animals lacking a PDGF ligand or receptor, we used rat and mouse whole-embryo culture (WEC) techniques to increase the exposure of embryos to the PDGF-AA or -BB ligands. Following a 48-hr culture period, we analyzed heart growth and cardiac myocyte differentiation. Exposure of rat embryos to 50 ng/ml of PDGF-AA resulted in a 42{\%} increase in total protein levels in the heart, but did not result in a significant increase in heart growth, as determined by measurements of the atrioventricular length and the left ventricular length and width. Exposure of embryos to 50 ng/ml of PDGF-BB resulted in a 77{\%} increase in total protein levels and a significant (P < 0.05) 8-15{\%} increase in the measured heart parameters. Although a comparison of control and PDGF-AA-treated embryos showed no increase in the overall size of the heart, confocal microscopy showed an increase in the size and number of myofibrillar bundles in the developing myocardium. In addition, transmission electron microscopy (TEM) revealed an increase in the presence of sarcomeres, indicating that myofibrils were more highly differentiated in these areas of the treated embryos. In PDGF-BB-treated embryos, the compact zone of the myocardium was thicker and, as shown by confocal microscopy and TEM, Lactin and well-developed sarcomeres were more prevalent, indicating that the myofibrils were more differentiated in the treated embryos than in the control embryos. These studies indicate that increased exposure of embryonic hearts to PDGF-AA or -BB increases the rate of myocardial development.",
keywords = "Cardiac development, Confocal microscopy, Electron microscopy, Myofibrillogenesis, Platelet-derived growth factor",
author = "Price, {Robert L.} and Haley, {Stephen T.} and Bullard, {Tara A.} and Goldsmith, {Edie C.} and Simpson, {David G.} and Thielen, {Thomas E.} and Yost, {Michael J.} and Louis Terracio",
year = "2003",
month = "5",
day = "1",
language = "English (US)",
volume = "272",
pages = "424--433",
journal = "Anatomical Record",
issn = "1932-8486",
publisher = "John Wiley and Sons Inc.",
number = "1",

}

TY - JOUR

T1 - Effects of platelet-derived growth factor-AA and -BB on embryonic cardiac development

AU - Price, Robert L.

AU - Haley, Stephen T.

AU - Bullard, Tara A.

AU - Goldsmith, Edie C.

AU - Simpson, David G.

AU - Thielen, Thomas E.

AU - Yost, Michael J.

AU - Terracio, Louis

PY - 2003/5/1

Y1 - 2003/5/1

N2 - Several studies have shown that disruption of the normal expression patterns of platelet-derived growth factor (PDGF) ligands and receptors during development results in gross cardiac defects and embryonic or neonatal death. However, little is known about the specific role that PDGF plays in the differentiation of cardiac myocytes. In experiments complementing studies that utilized naturally-occurring Patch mice lacking the PDGFr α, or knockout animals lacking a PDGF ligand or receptor, we used rat and mouse whole-embryo culture (WEC) techniques to increase the exposure of embryos to the PDGF-AA or -BB ligands. Following a 48-hr culture period, we analyzed heart growth and cardiac myocyte differentiation. Exposure of rat embryos to 50 ng/ml of PDGF-AA resulted in a 42% increase in total protein levels in the heart, but did not result in a significant increase in heart growth, as determined by measurements of the atrioventricular length and the left ventricular length and width. Exposure of embryos to 50 ng/ml of PDGF-BB resulted in a 77% increase in total protein levels and a significant (P < 0.05) 8-15% increase in the measured heart parameters. Although a comparison of control and PDGF-AA-treated embryos showed no increase in the overall size of the heart, confocal microscopy showed an increase in the size and number of myofibrillar bundles in the developing myocardium. In addition, transmission electron microscopy (TEM) revealed an increase in the presence of sarcomeres, indicating that myofibrils were more highly differentiated in these areas of the treated embryos. In PDGF-BB-treated embryos, the compact zone of the myocardium was thicker and, as shown by confocal microscopy and TEM, Lactin and well-developed sarcomeres were more prevalent, indicating that the myofibrils were more differentiated in the treated embryos than in the control embryos. These studies indicate that increased exposure of embryonic hearts to PDGF-AA or -BB increases the rate of myocardial development.

AB - Several studies have shown that disruption of the normal expression patterns of platelet-derived growth factor (PDGF) ligands and receptors during development results in gross cardiac defects and embryonic or neonatal death. However, little is known about the specific role that PDGF plays in the differentiation of cardiac myocytes. In experiments complementing studies that utilized naturally-occurring Patch mice lacking the PDGFr α, or knockout animals lacking a PDGF ligand or receptor, we used rat and mouse whole-embryo culture (WEC) techniques to increase the exposure of embryos to the PDGF-AA or -BB ligands. Following a 48-hr culture period, we analyzed heart growth and cardiac myocyte differentiation. Exposure of rat embryos to 50 ng/ml of PDGF-AA resulted in a 42% increase in total protein levels in the heart, but did not result in a significant increase in heart growth, as determined by measurements of the atrioventricular length and the left ventricular length and width. Exposure of embryos to 50 ng/ml of PDGF-BB resulted in a 77% increase in total protein levels and a significant (P < 0.05) 8-15% increase in the measured heart parameters. Although a comparison of control and PDGF-AA-treated embryos showed no increase in the overall size of the heart, confocal microscopy showed an increase in the size and number of myofibrillar bundles in the developing myocardium. In addition, transmission electron microscopy (TEM) revealed an increase in the presence of sarcomeres, indicating that myofibrils were more highly differentiated in these areas of the treated embryos. In PDGF-BB-treated embryos, the compact zone of the myocardium was thicker and, as shown by confocal microscopy and TEM, Lactin and well-developed sarcomeres were more prevalent, indicating that the myofibrils were more differentiated in the treated embryos than in the control embryos. These studies indicate that increased exposure of embryonic hearts to PDGF-AA or -BB increases the rate of myocardial development.

KW - Cardiac development

KW - Confocal microscopy

KW - Electron microscopy

KW - Myofibrillogenesis

KW - Platelet-derived growth factor

UR - http://www.scopus.com/inward/record.url?scp=0141960054&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0141960054&partnerID=8YFLogxK

M3 - Article

C2 - 12704700

AN - SCOPUS:0141960054

VL - 272

SP - 424

EP - 433

JO - Anatomical Record

JF - Anatomical Record

SN - 1932-8486

IS - 1

ER -